鱼类发育与生物技术
学科带头人：孙永华 研究员
学科组成员：
朱作言 研究员、院士、孙永华 研究员、叶鼎 副研究员、何牡丹 项目副研、王厚鹏 实验师
联系方式：
027-68780235（孙永华办）；027-68780935（实验室）
学科组介绍：
高效繁育和优良品种是可持续发展水产养殖的前提，鱼类发育与生物技术学科组致力于解决高效繁育的鱼类生殖细胞发育研究和培育优良品种的前沿育种生物技术研发。
学科组的主要研究兴趣包括：
1） 鱼类生殖干细胞命运决定和分化、配子发生、胚胎发育的分子和细胞学机制研究；
2） 稳定高效的基因编辑和借腹生殖等鱼类前沿育种生物技术的研发；
3） 优质、高产的养殖鱼类新种质的研制和快速定向育种。
学科组已建立一整套成熟的遗传学、细胞生物学、生物化学、生物影像学、多组学分析等多元化的研究技术平台，在鱼类生殖细胞与胚胎发育的育种基础理论研究、规模化遗传和育种模型构建、基因编辑配子“借腹生殖”前沿育种生物技术研发等方面取得重要进展。学科组在 Genome Research、PLOS Biology、Nature Communications、PLOS Genetics、Journal of Biological Chemistry、Endocrinology、Cell Research、Science Bulletin等国内外学术刊物上发表论文100 余篇。
学科组目前承担有“发育编程及其代谢调节”国家重点研发计划重点专项、国家杰出青年科学基金、国家自然科学基金创新研究群体、国家自然科学基金面上项目、中科院先导专项等一批科研项目。
近三年发表的主要论文（* 示通讯作者）：
1) Zhang, F., Hao, Y., Li, X., Li, Y., Ye, D., Zhang, R., Wang, X., He, M., Wang, H., Zhu, Z., and Sun, Y.* (2022). Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation. Sci China Life Sci 65, 969-987. 10.1007/s11427-021-1989-9.
2) Wang, Y., Ye, D., Zhang, F., Zhang, R., Zhu, J., Wang, H., He, M., and Sun, Y.* (2022). Cyp11a2 Is Essential for Oocyte Development and Spermatogonial Stem Cell Differentiation in Zebrafish. Endocrinology 163. 10.1210/endocr/bqab258.
3) Ye, D., and Sun, Y.* (2021). Manipulating and Visualizing the Germline with Transgenic Lines. Methods Mol Biol 2218, 265-276. 10.1007/978-1-0716-0970-5_21.
4) Mohammed, Y., Ye, D., He, M., Wang, H., Li, Y., Han, D., Zhu, Z., and Sun, Y.* (2021). Induction of biosynthesis of ketocarotenoid from β-carotene in fish embryos. Aquaculture 542, 736863. 10.1016/j.aquaculture.2021.736863.
5) He, M., Zhang, R., Jiao, S., Zhang, F., Ye, D., Wang, H., and Sun, Y.* (2020). Nanog safeguards early embryogenesis against global activation of maternal β-catenin activity by interfering with TCF factors. PLOS Biology 18, e3000561.
6) Sun, Y., Zhang, B., Luo, L., Shi, D.L., Wang, H., Cui, Z., Huang, H., Cao, Y., Shu, X., Zhang, W., et al. (2020). Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9. Genome Research 30, 118–126.
7) Li, X., Zhang, F., Wu, N., Ye, D., Wang, Y., Zhang, X., Sun, Y., and Zhang, Y.A. (2020). A critical role of foxp3a-positive regulatory T cells in maintaining immune homeostasis in zebrafish testis development. J Genet Genomics 47, 547-561. 10.1016/j.jgg.2020.07.006.（封面论文）
8) Zhang, Q., Ye, D., Wang, H., Wang, Y., Hu, W., and Sun, Y.* (2020). Zebrafish cyp11c1 Knockout Reveals the Roles of 11-ketotestosterone and Cortisol in Sexual Development and Reproduction. Endocrinology 161, DOI:10.1210/endocr/bqaa048 （2020、2021年度高被引论文）
9) Zhang, F., Li, X., He, M., Ye, D., Xiong, F., Amin, G., Zhu, Z., and Sun, Y.* (2020). Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells. Journal of Genetics and Genomics 47, 37-47. (Faculty of 1000推荐论文)
10) Ye, D., Wang, X., Wei, C., He, M., Wang, H., Wang, Y., Zhu, Z., and Sun, Y.* (2019). Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLOS Genetics 15, e1008306.
11) Ye, D., Zhu, L., Zhang, Q., Xiong, F., Wang, H., Wang, X., He, M., Zhu, Z., and Sun, Y.* (2019). Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline. Mar Biotechnol (NY) 21, 217-228. (Springer Nature 2019 Highlights论文)
12) Sun, Y.*, and Zhu, Z. (2019). Designing future farmed fishes using genome editing. Sci China Life Sci 62, 420-422. 10.1007/s11427-018-9467-x.
