鱼类免疫与寄生虫--中国科学院水生生物研究所

水产抗逆与病害防控研究室

鱼类免疫与寄生虫

文本大小：【大 | 中 | 小】

学科带头人：聂品 (Nie, Pin)

学科组介绍：
鱼类免疫学与寄生虫学学科组主要从事低等脊椎动物免疫学和病原生物学方面的基础研究，发展可用于水产动物病害防治的疫苗研制的理论和技术，近期的研究活动主要在于：1）鱼类免疫器官和免疫细胞的组成、个体发生，特异和非特异性免疫分子的基因、纯化以及功能，鱼类对病原的免疫反应规律；2）以鱼类寄生虫和血吸虫为对象，研究寄生后生动物的致病机理、种群生态及其与宿主的协同进化；3）鱼类细菌性病原的毒力因子、毒力的鉴定与致病机理，疫苗侯选基因的筛选，毒力因子基因缺失突变株的构建。学科组负责人聂品研究员为湖北省有突出贡献中青年专家，新世纪国家百千万人才工程入选者。固定人员包括：谢海侠研究员、李楠博士和姚卫建高级实验师。

联系方式：027 68780736, 027 68780005，Email: pinnie@ihb.ac.cn
地点：新实验大楼504-509

仪器设备：Nikon解剖镜, Olympus显微镜, PCR仪, 冷冻离心机, 双向电泳系统, 凝胶成像系统, 杂交箱, 蛋白纯化仪, 蛋白测序仪。

近三年发表文章：

Zhang S H; Zhao Q P; Jiao R; Gao Q; Nie P.Identification of polymorphic microsatellites for the intermediate host Oncomelania hupensis of Schistosoma japonicum in China.Malacologia (in press).
Xu Q Q; Chang M X; Sun R H; Xiao F S; Nie P. The first non-mammalian CXCR5 in a teleost fish: molecular cloning and expression analysis in grass carp (Ctenopharyngodon idella). BMC immunology 2010 (in press).
Huang B; Qi Z T; Xu Z; Nie P.Global characterization of interferon regulatory factor (IRF) genes in vertebrates: Glimpse of the diversification in evolution.BMC immunology 2010; 11, 22.
Chen D L; Guo X G; Nie P.Phylogenetic studies of sinipercid fish (Perciformes: Sinipercidae) based on multiple genes, with first application of an immune-related gene, the virus-induced protein (viperin) gene.Molecular phylogenetics and evolution 2010; 55, 1167-1176.
Qi Z T; Nie P; Secombes C J; Zou J.Intron-containing Type I and Type III IFN coexist in amphibians: Refuting the concept that a retroposition event gave rise to Type I IFNs.Journal of immunology 2010; 184, 5038-5046.
Zhao Q P; Jiang M S; Littlewood D T J; Nie P.Distinct genetic diversity of Oncomelania hupensis, intermediate host of Schistosoma japonicum in mainland China as revealed by ITS sequences.PLoS neglected tropical diseases 2010; 4: e611.
Li N; Zhang J; Zhang L Q; Nie P. Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnareby using suppression subtractive hybridization. Journal of fish diseases 2010; 33(5):403-12.
Xu Qiao Q; Chang Ming X; Xiao Fan S; Huang Bei; Nie P. The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella. Veterinary immunology and immunopathology 2010; 134(3-4):269-78.
Chen W Q; Xu Q Q; Chang M X; Zou J; Secombes C J; Peng K M; Nie P. Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gammarel) in grass carp Ctenopharyngodon idella. Veterinary immunology and immunopathology 2010; 134(3-4):199-207.
Chen W Q; Xu Q Q; Chang M X; Nie P; Peng K M. Molecular characterization and expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella. Fish & shellfish immunology 2010; 28(1):18-29.
Chang M X; Chen W Q; Nie P. Structure and expression pattern of teleost caspase recruitment domain (CARD) containing proteins that are potentially involved in NF-kappaB signalling. Developmental and comparative immunology 2010; 34(1):1-13.
Tian J Y; Xie H X; Zhang Y A; Xu Z; Yao W J; Nie P. Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsiidentified by in situ hybridisation. Veterinary immunology and immunopathology 2009; 132(2-4):146-52.
Xiong Fan; Wang Gui Tang; Wu Shan Gong; Nie P. Development of Eustrongylidesignotus(Nematoda: Dioctophmida) in domestic ducks (Anas platyrhynchosdomestica(L.)). The Journal of parasitology 2009; 95(5):1035-9.
Xu Z; Chang M X; Qi Z T; Huang B; Nie P. Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis). Veterinary immunology and immunopathology 2009; 131(3-4):268-72.
Gao Q; Nie P; Thompson K D; Adams A; Wang T; Secombes C J; Zou J. The search for the IFN-gamma receptor in fish: Functional and expression analysis of putative binding and signalling chains in rainbow trout Oncorhynchus mykiss. Developmental and comparative immunology 2009; 33(8):920-31.
Xu Zhen; Wang Gai L; Nie P. IgM, IgD and IgY and their expression pattern in the Chinese soft-shelled turtle Pelodiscus sinensis. Molecular immunology 2009; 46(10):2124-32.
Chang Mingxian; Nie P; Collet Bertrand; Secombes Christopher J; Zou J. Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykissprovides evidence of a major gene duplication event within this gene family in teleosts. Immunogenetics 2009; 61(4):315-25.
Chang M X; Wang Y P; Nie P. Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways. Fish & shellfish immunology 2009; 26(2):264-74.
Liu Y; Chang M X; Wu S G; Nie P. Characterization of C-C chemokine receptor subfamily in teleost fish. Molecular immunology 2009; 46(3):498-504.
Zou Jun; Chang Mingxian; Nie P; Secombes Chris J. Origin and evolution of the RIG-I like RNA helicase gene family. BMC evolutionary biology 2009; 9:85.
Li Wen X; Nie P; Wang Gui T; Yao Wei J. Communities of gastrointestinal helminths of fish in historically connected habitats: habitat fragmentation effect in a carnivorous catfish Pelteobagrus fulvidracofrom seven lakes in flood plain of the Yangtze River, China. Parasites & vectors 2009; 2(1):22.
Qi Z T; Nie P. Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis. Immunogenetics 2008; 60(11):699-710.
Chang M X; Nie P. RNAi suppression of zebrafish peptidoglycan recognition protein 6 (zfPGRP6) mediated differentially expressed genes involved in Toll-like receptor signaling pathway and caused increased susceptibility to Flavobacterium columnare. Veterinary immunology and immunopathology 2008; 124(3-4):295-301.
Xu Z Y; Nie P; Chang M X; Sun B J. Cloning, characterization and expression analysis of SIMP (source of immunodominant MHC-associated peptides) in grass carp Ctenopharyngodon idella. Fish & shellfish immunology 2008; 24(6):701-14.
Liu G Y; Nie P; Zhang J; Li N. Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Flavobacterium columnare. Journal of fish diseases 2008; 31(4):269-76.
Gao Dian; Wang Gui Tang; Xi Bing Wen; Yao Wei Jian; Nie P. A new species of Allocreadium(Trematoda: Allocreadiidae) from freshwater fishes in the Danjiangkou Reservoir in China. The Journal of parasitology 2008; 94(1):176-80.
Song Y; Wang G T; Yao W J; Gao Q; Nie P. Phylogeny of freshwater parasitic copepods in the Ergasilidae (Copepoda: Poecilostomatoida) based on 18S and 28S rDNA sequences. Parasitology research 2008; 102(2):299-306.
Xu Z Y; Sun B J; Chang M X; Nie P. Characterization and expression analysis of TNFR-associated factor 1 (TRAF1) in grass carp Ctenopharyngodon idella. Veterinary immunology and immunopathology 2008; 121(1-2):44-57.
Harun Nor Omaima; Zou Jun; Zhang Yang-An; Nie P; Secombes Chris J. The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8. Developmental and comparative immunology 2008; 32(6):673-81.
Xu Zaiyan; Nie P; Sun Baojian; Chang M X. Molecular identification and expression analysis of tumor necrosis factor receptor-associated factor 2 in grass carp Ctenopharyngodon idella. Acta biochimica et biophysica Sinica 2007; 39(11):857-68.
Li L; Chang M X; Nie P. Molecular cloning, promoter analysis and induced expression of the complement component C9 gene in the grass carp Ctenopharyngodon idella. Veterinary immunology and immunopathology 2007; 118(3-4):270-82.
Chen Dali; Wang Guitang; Yao Weijian; Nie P. Utility of ITS1-5.8S-ITS2 sequences for species discrimination and phylogenetic inference of two closely related bucephalid digeneans (Digenea: Bucephalidae): Dollfustrema vaneyiand Dollfustrema hefeiensis. Parasitology research 2007; 101(3):791-800.
Chang M X; Nie P. Intelectin gene from the grass carp Ctenopharyngodon idella: cDNA cloning, tissue expression, and immunohistochemical localization. Fish & shellfish immunology 2007; 23(1):128-40.
Sun B J; Xie H X; Song Y; Nie P. Gene structure of an antimicrobial peptide from mandarin fish, Siniperca chuatsi(Basilewsky), suggests that moronecidins and pleurocidins belong in one family: the piscidins. Journal of fish diseases 2007; 30(6):335-43.
Wu S G; Wang G T; Xi B W; Gao D; Nie P. Population dynamics and maturation cycle of Camallanus cotti(Nematoda: Camallanidae) in the Chinese hooksnout carp Opsariichthys bidens(Osteichthyes: Cyprinidae) from a reservoir in China. Veterinary parasitology 2007; 147(1-2):125-31.
Gao Q; X Chen M; Yao W J; Gao Y; Song Y; Wang G T; Wang M X; Nie P. Phylogeny of diplozoids in five genera of the subfamily Diplozoinae Palombi, 1949 as inferred from ITS-2 rDNA sequences. Parasitology 2007; 134(Pt 5):695-703.
Chang M X; Nie P; Wei L L. Short and long peptidoglycan recognition proteins (PGRPs) in zebrafish, with findings of multiple PGRP homologs in teleost fish. Molecular immunology 2007; 44(11):3005-23.
Sun B J; Chang M X; Song Y; Yao W J; Nie P. Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi. Veterinary immunology and immunopathology 2007; 116(1-2):26-36.
Chang M X; Sun B J; Nie P. The first non-mammalian CXCR3 in a teleost fish: gene and expression in blood cells and central nervous system in the grass carp (Ctenopharyngodon idella). Molecular immunology 2007; 44(6):1123-34.

　　　　　　　　　 (last updated 9th May, 2010)

== 实验室与学会 ==

== 实验室与学会 ==
水产品种创制与高效养殖全国重点实验室
中国科学院藻类生物学重点实验室
农业部淡水养殖病害防治重点实验室
武汉白暨豚保护基金会
湖北省海洋湖沼学会
中国动物学会原生动物学分会
中国动物学会斑马鱼分会
湖北省暨武汉动物学会
中国水产学会鱼病学专业委员会
中国鱼类学会

== 平台建设 ==

== 平台建设 ==
“一带一路”海域赤潮数据库
国家水生生物种质资源库
国家斑马鱼资源中心
中国科学院淡水藻种库
中国科学院武汉生命科学大型仪器区域中心
湿地生态系统观测研究野外站联盟
中国科学院水生生物研究所分析测试中心
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水生生物博物馆

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