研究组组长：昌鸣先 研究员 

　　研究组成员：张杰 副研究员；武小曼 助理研究员；在读研究生11人

　　研究组介绍：鱼类比较免疫学与健康养殖学研究组致力于鱼类免疫学、病原与宿主相互作用的分子机理、病原感染的免疫应答机制及病原清除机制、水生经济动物疾病的发生、发展机理与免疫防控技术的研究。

近期正在开展的研究工作包括：

1）抗病抗逆鱼新种质创制;

2）天然免疫识别的比较与进化研究：主要研究介导天然免疫识别的受体及其信号通路上的关键分子在进化中的功能差异;

3）鱼类代谢、免疫与病原三者间的互作机理：主要研究鱼类代谢与免疫、病原入侵与感染之间的相互调控关系，鱼类免疫-代谢途径中关键分子的发掘及其在感染性疾病中的功能;

4）从水生经济动物、病原体和养殖环境三者之间的关系入手，开展重要疾病的发生发展和流行规律研究、环境调控对病原和机体免疫功能的影响以及免疫学防控技术的研究。

研究组在Reviews in Aquaculture、PLOS Pathogens、Communications Biology、Frontiers in Immunology、Immunology、Journal of Virology、Journal of Immunology、Ecological Indicators、Environmental Microbiology、Aquaculture、Cell Res、Protein Cell等国内外学术刊物上发表论文100 余篇；目前承担有国家重点研发计划、国家科技重大专项、国家自然科学基金联合基金项目重点支持项目、A类先导专项、B类先导专项等重要项目。

联系方式：Email: mingxianchang@ihb.ac.cn

近年发表的部分论文（*通讯作者）：

1.     Li PW, Zhang J, Chang MX*. Structure, function and immune evasion strategies of aquareoviruses,with focus on grass carp reovirus. Rev Aquac. 2024;16(1):410-432.

2.     Fang H, Wu XM, Zheng SY, Chang MX*. Tripartite motif 2b (trim2b) restricts spring viremia of carp virus by degrading viral proteins and negative regulators NLRP12-like receptors. J Virol. 2024;98(6):e0015824.

3.     Song YJ, Zhang J, Xu Z, Nie P, Chang MX*. Liver X Receptor LXRα Promotes Grass Carp Reovirus Infection by Attenuating IRF3-CBP Interaction and Inhibiting RLR Antiviral Signaling. J Immunol. 2023;211(6):1006-1019.

4.     Song YJ, Zhang J, Xiao J, Feng H, Xu Z, Nie P, Chang MX*. Piscine Vitamin D Receptors Vdra/Vdrb in the Absence of Vitamin D Are Utilized by Grass Carp Reovirus for Promoting Viral Replication. Microbiol Spectr. 2023;11(4):e0128723.

5.     Chang MX.* Emerging mechanisms and functions of inflammasome complexes in teleost fish. Front Immunol. 2023;14:1065181.

6.     Zhang J, Chang MX*. TBK1 Isoform Inhibits Grass Carp Reovirus Infection by Targeting the Degradation of Viral Nonstructural Proteins NS80 and NS38. J Immunol. 2023;210(2):191-203.

7.     Zhang J, Li P, Lu R, Ouyang S*, Chang MX*. Structural and functional analysis of the small GTPase ARF1 reveals a pivotal role of its GTP-binding domain in controlling of the generation of viral inclusion bodies and replication of grass carp reovirus. Front Immunol. 2022;13:956587.

8.     Cao L, Fang H, Yan D, Wu XM, Zhang J, Chang MX*. CD44a functions as a regulator of p53 signaling, apoptosis and autophagy in the antibacterial immune response. Commun Biol. 2022;5(1):889.

9.     Fang H, Yang YY, Wu XM, Zheng SY, Song YJ, Zhang J, Chang MX*. Effects and Molecular Regulation Mechanisms of Salinity Stress on the Health and Disease Resistance of Grass Carp. Front Immunol. 2022;13:917497.

10.  Yang YY, Zheng SY, Fang H, Wu XM, Zhang J, Chang MX*. Immunoprotective Effects of Two Histone H2A Variants in the Grass Carp Against Flavobacterium columnare Infection. Front Immunol. 2022;13:939464.

11.  Zhang J, Wu XM, Fang Q, Bi YH, Nie P, Chang MX*. Grass Carp Reovirus Nonstructural Proteins Avoid Host Antiviral Immune Response by Targeting the RLR Signaling Pathway. J Immunol. 2022;208(3):707-719.

12.  Xiong F, Cao L, Xiong J, Wu YF, Huang WS, Chang MX*. Time-resolved and multi-tissue RNAseq provides new insights on the immune responses of European eels following infection with Aeromonas hydrophila. Water Biology and Security. 2022,1: 100003.

13.  Wu XM, Fang H, Zhang J, Bi YH, Chang MX*. Histone H2A Nuclear/Cytoplasmic Trafficking Is Essential for Negative Regulation of Antiviral Immune Response and Lysosomal Degradation of TBK1 and IRF3. Front Immunol. 2021;12:771277.

14.  Cao L, Yan D, Xiao J, Feng H, Chang MX*. The Zebrafish Antiapoptotic Protein BIRC2 Promotes Edwardsiella piscicida Infection by Inhibiting Caspases and Accumulating p53 in a p53 Transcription-Dependent and -Independent Manner. Front Immunol. 2021;12:781680.

15.  Qi Z*, Yan D, Cao L, Xu Y, Chang M*. Zebrafish BID Exerts an Antibacterial Role by Negatively Regulating p53, but in a Caspase-8-Independent Manner. Front Immunol. 2021;12:707426.

16.  Chang MX*, Xiong F, Wu XM, Hu YW. The expanding and function of NLRC3 or NLRC3-like in teleost fish: Recent advances and novel insights. Dev Comp Immunol. 2021;114:103859.

17.  Wu X, Xiong F, Fang H, Zhang J, Chang M*. Crosstalks between NOD1 and Histone H2A Contribute to Host Defense against Streptococcus agalactiae Infection in Zebrafish. Antibiotics (Basel). 2021;10(7):861.

18.  Li P, Chang M*. Roles of PRR-Mediated Signaling Pathways in the Regulation of Oxidative Stress and Inflammatory Diseases. Int J Mol Sci. 2021;22(14):7688.

19.  Xiong F, Cao L, Wu XM, Chang MX*. The function of zebrafish gpbar1 in antiviral response and lipid metabolism. Dev Comp Immunol. 2021;116:103955.

20.  Wu XM^#, Zhang J^#, Li PW, Hu YW, Cao L, Ouyang S, Bi YH, Nie P, Chang MX*. NOD1 Promotes Antiviral Signaling by Binding Viral RNA and Regulating the Interaction of MDA5 and MAVS. J Immunol. 2020;204(8):2216-2231.

21.  Zhang J, Wu XM, Hu YW, Chang MX*. A Novel Transcript Isoform of TBK1 Negatively Regulates Type I IFN Production by Promoting Proteasomal Degradation of TBK1 and Lysosomal Degradation of IRF3. Front Immunol. 2020;11:580864.

22.  Chang MX*, Xiong F, Wu XM, Hu YW. The expanding and function of NLRC3 or NLRC3-like in teleost fish: Recent advances and novel insights. Dev Comp Immunol. 2020;114:103859.

23.  Chang MX*, Xiong F. Astaxanthin and its Effects in Inflammatory Responses and Inflammation-Associated Diseases: Recent Advances and Future Directions. Molecules. 2020;25(22):E5342.

24.  Xiong F, Cao L, Wu XM, Chang MX*. The function of zebrafish gpbar1 in antiviral response and lipid metabolism. Dev Comp Immunol. 2020;116:103955.

25.  Fang H, Wu XM, Hu YW, Song YJ, Zhang J, Chang MX. NLRC3-like 1 inhibits NOD1-RIPK2 pathway via targeting RIPK2. Dev Comp Immunol. 2020;112:103769.

26.  Xiong F, Xiong J, Wu YF, Cao L, Huang WS*, Chang MX*. Time-resolved RNA-seq provided a new understanding of intestinal immune response of European eel (Anguilla anguilla) following infection with Aeromonas hydrophila. Fish Shellfish Immunol. 2020;105:297-309.

27.  Nabi G, McLaughlin RW, Khan S, Hao Y, Chang MX. Pneumonia in endangered aquatic mammals and the need for developing low-coverage vaccination for their management and conservation. Anim Health Res Rev. 2020:1-9.

28.  武小曼，方红，聂品，昌鸣先*. 鱼类组蛋白核苷酸的多态性及其在杀鱼爱德华氏菌感染中的作用. 水产学报, 2020, 44(0): 1−14.

29.  Zhang C, Zhang Q, Wang J, Tian J, Song Y, Xie H, Chang M, Nie P, Gao Q*, Zou J*. Transcriptomic responses of S100 family to bacterial and viral infection in zebrafish. Fish Shellfish Immunol. 2019;94:685-696.

30.  Wu XM, Cao L, Nie P, Chang MX*. Histone H2A cooperates with RIP2 to induce the expression of antibacterial genes and MHC related genes. Dev Comp Immunol. 2019;101:103455.

31.  Wu XM, Cao L, Hu YW, Chang MX*. Transcriptomic characterization of adult zebrafish infected with Streptococcus agalactiae. Fish Shellfish Immunol. 2019;94:355-372.

32.  Cao L, Wu XM, Nie P, Chang MX*. The negative regulation of piscine CD44c in viral and bacterial infection. Dev Comp Immunol. 2019;96:135-143.

33.  Rahman K, Khan SU, Fahad S*, Chang MX*, Abbas A, Khan WU, Rahman L, Haq ZU, Nabi G, Khan D. Nano-biotechnology: a new approach to treat and prevent malaria. Int J Nanomedicine. 2019;14:1401-1410.

34.  Zhang J, Guo H, Zhang F, Chen Q, Chang M*, Fang Q*. NS38 is required for aquareovirus replication via interaction with viral core proteins and host eIF3A. Virology. 2019;529:216-225.

35.  Zhen X, Zhou H, Ding W, Zhou B, Xu X, Perčulija V, Chen CJ, Chang MX, Choudhary MI, Ouyang S*. Structural basis of AimP signaling molecule recognition by AimR in Spbeta group of bacteriophages. Protein Cell. 2019;10(2):131-136.

36.  Wu XM, Chen WQ, Hu YW, Cao L, Nie P, Chang MX*. RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways. Front Immunol. 2018;9:726.

37.  Cao L, Wu XM, Hu YW, Xue NN, Nie P, Chang MX*. The discrepancy function of NLRC5 isoforms in antiviral and antibacterial immune responses. Dev Comp Immunol. 2018;84:153-163.

38.  Hu YW, Zhang J, Wu XM, Cao L, Nie P, Chang MX*. TANK-Binding Kinase 1 (TBK1) Isoforms Negatively Regulate Type I Interferon Induction by Inhibiting TBK1-IRF3 Interaction and IRF3 Phosphorylation. Front Immunol. 2018;9:84.

39.  Zhang B, Ye W, Ye Y, Zhou H, Saeed AFUH, Chen J, Lin J, Perčulija V, Chen Q, Chen CJ, Chang MX, Choudhary MI, Ouyang S*. Structural insights into Cas13b-guided CRISPR RNA maturation and recognition. Cell Res. 2018;28(12):1198-1201.

40.  Chang MX^#, Zou J^#, Nie P, Huang B, Yu Z, Collet B, Secombes CJ*. Intracellular interferons in fish: a unique means to combat viral infection. PLoS Pathog. 2013;9(11):e1003736.