鱼类生理生态学
学科带头人:解绶启
学科组成员:解绶启 朱晓鸣 韩冬 杨云霞 金俊琰 刘昊昆 聂光汉
学科组介绍:
鱼类生理生态学学科组主要研究水产养殖动物生理生态学特征,探讨其与环境相关的营养学和饲料技术等,为集约化养殖及渔业管理等提供有关营养、饲料、投喂、相关环境因子作用等基础理论和技术。
近期主要研究领域包括:
1. 基础营养学及能量学:包括营养需求、饲料原料利用、动物生长的环境及内在调控机制等,探讨动物营养物代谢的遗传背景及分子调控机制。
2. 精准营养配给理论与技术:研究动物的不同条件下的精准营养需求和动态投喂模型,为提高养殖效率和产品品质、建立可持续渔业模式提供理论与技术。
3. 比较生理生态学:研究鱼类的营养能量学特征等与其食性进化的关系,为建立鱼类营养需求和饲料利用的评价模型提供基础。
4. 饲料营养与水产品品质:研究饲料营养组成及投喂对水产动物品质的影响,探讨饲料源有毒有害物质的限量,为改善动物福利、评价饲料卫生安全、保障食品安全、提高产品品质提供科学依据。
仪器设备:
鱼类生理生态学研究室是淡水生态与生物技术国家重点实验室(FEBL)的一部分,主要从事鱼类营养学、能量学及淡水渔业的相关基础理论和应用研究。
鱼类生理生态学研究室的分析实验室主要包括生化分析实验室、分子生物学实验室和细胞培养实验室。
生化分析实验室:拥有生化分析实验室必备的器皿、试剂以及分析条件。可进行常规生物、化学分析工作,现有仪器:
1、 绝热式微量氧弹热量计:美国Parr公司,主要用于样品的能值测定;
2、 自平衡式氧弹热量计:美国Parr公司,主要用于样品的能值测定;
3、 自动定氮仪:丹麦FOSS,可测定固体和液体样品的含氮量;
4、 脂肪抽提仪:丹麦FOSS,测定样品粗脂肪含量;
5、 氨基酸分析仪:日本HITACHI,测定样品氨基酸含量;
6、 在高压蒸气灭菌器,日本TOMY KOGYO公司,用于样品处理、试剂制备以及实验器材的消毒;
7、 马福炉:国产,测定样品灰分含量;
8、 全自动能量收支同步测定仪:10套,计算机控制,包括代谢室、测氧仪、放大器和计算机接口等的流水、控温、测氧和记录等功能;
9、 鱼类活动监测系统:美国进口,包括摄像、显示监测、数据转换和数据输出系统,用于监测鱼类的活动;
10、溶氧仪:美国YSI公司,用于测定养殖水体中溶氧含量;
11、 酶标仪,美国Bio-Tek,主要用于终点法ELISA、动力学法ELISA/酶学分析、细胞增殖/细胞毒性、微生物生长/MIC、蛋白质定量分析;
12、 分光光度计:T6系列紫外可见分光光度计,北京普析通用仪器有限责任公司,用于水化学分析测定、生物样品测定等;
13、 天平:德国Sartorius 公司,瑞士Mettler Toledo公司,微量天平一台、半微量天平两台、分析天平四台,用于样品分析,试剂配制等工作;
14、 离心机:德国Eppendorf公司,MiniSpin?、5418 、5702、5701R、5810R等小中大型、常温以及冷冻离心机,用于样品的处理、分析。
分子生物学实验室和细胞培养实验室:主要进行分子生物学和细胞生物学的相关操作以及样品的分析以及检测,现有仪器。
1、 高通量匀浆器:MP*The FastPrep-24动植物组织均质器,最先进、快速及高效提取样品中的DNA、RNA 和蛋白质;
2、 实时荧光定量PCR仪,德国罗氏LightCycler 480Ⅱ,用于基因表达分析研究,SNP单核苷酸多态性和突变位点的分析检测,包括绝对定量、相对定量、基因分型(熔解曲线法及终点法)、高分辨率熔解曲线分析等;
3、 超微量分光光度计:Thermo Nanodrop8000,用于核酸和蛋白等微量样品的紫外/可见光检测;
4、 电泳系统:美国Bio-rad电泳仪及电泳槽,用于凝胶电泳。
5、 UVP凝胶成像系统:美国UVP公司,DNA/RNA/蛋白质等凝胶电泳不同染色(如eb、考马氏亮蓝、银染、sybr green)及微孔板、平皿等非化学发光成像检测分析。凝胶成像系统可以应用于分子量计算,密度扫描,密度定量, PCR定量等生物工程常规研究;
6、 超净工作台:进行细胞生物学操作、保护在工作台内操作的试剂和样品等不受污染;
7、 细胞培养箱:德国MEMMERT,用于组织、细胞培养;
8、 -80℃超低温冰箱:日本三洋、日本松下,用于生物样品、试剂的的保存;
9、 4℃、-20℃冰箱,博世,用于生物样品、试剂的保存;
10、 天平、离心机、恒温摇床、移液器、恒温培养箱若干。
近三年发表文章:
1. Binyuan Yang, Gang Zhai, Yulong Gong, Jingzhi Su, Dong Han*, Zhan Yin, Shouqi Xie, Depletion of insulin receptors leads to b-cell hyperplasia in zebrafish. Science Bulletin, (Online)
2. Junyan Jin, Xiaoming Zhu, Dong Han, Yunxia Yang, Haokun Liu, Shouqi Xie*. Different regulation of insulin on glucose and lipid metabolism in 2 strains of gibel carp. ?General and Comparative Endocrinology. 2017. DOI: 10.1016/j.ygcen.2017.01.012.
3. Yulong Gong, Weijun Chen, Dong Han*, Xiaoming Zhu, Yunxia Yang , Junyan Jin, Haokun Liu, Shouqi Xie. Effects of food restriction on growth, body composition and gene expression related in regulation of lipid metabolism and food intake in grass carp. Aquaculture,2017. 469:28-35.
4. 唐启升,韩冬, 毛玉泽, 张文兵, 单秀娟。中国水产养殖种类组成、不投饵率和营养级。中国水产科学,2016, 23(4): 729-758。
5. 解绶启, 张文兵,韩冬,麦康森. 水产养殖动物营养与饲料工程发展战略研究。中国工程科学,2016 ,18(3):29-36.
6. 解绶启,薛敏。水产养殖动物摄食及其营养调控。中国水产,2016(1):103-105.
7. Dongdong Xie, Dong Han, Xiaoming Zhu, Yunxia Yang, Junyan Jin, Haokun Liu & Shouqi Xie*.Dietary available phosphorus requirement of on-growing gibel carp (Carassius auratus gibelio var. CAS III). Aquaculture Nutrition (DOI: 10.1111/anu.12478)
8. Ling Zhu, Dong Han, Xiaoming Zhu, Yunxia Yang, Junyan Jin, Haokun Liu & Shouqi Xie*. Dietary selenium requirement for on-growing gibel carp(Carassius auratus gibelio var. CAS III). Aquaculture Research (doi:10.1111/are.13118)
9. Dong Han, Xiujuan Shan,Wenbing Zhang, Yushun Chen, Qingyin Wang, Zhongjie Li, Guofan Zhang, Pao Xu, Jiale Li, Shouqi Xie, Kangsen Mai, Qisheng Tang* and Sena S. De Silva. A revisit to fishmeal usage and associated consequences in Chinese aquaculture. Reviews in Aquaculture (2016) (doi: 10.1111/raq.12183)
10. Liye Shao, Xiaoming Zhu, Yunxia Yang, Junyan Jin, Haokun Liu, Dong Han*, Shouqi Xie. 2016. Effects of dietary vitamin A on growth, hematology, digestion and lipometabolism of on-growing gibel carp (Carassius auratus gibelio var. CAS III). Aquaculture . 460:83-89.
11. Wenjun Ye, Dong Han, Xiaoming Zhu, Yunxia Yang, Junyan Jin, Shouqi Xie*. Comparative study on dietary protein requirements for juvenile and pre-adult gibel carp (Carassius auratus gibelio var. CAS III). Aquaculture Nutrition (DOI: 10.1111/anu.12442)
12. Haokun Liu, Junyan Jin,Xiaoming Zhu, Dong Han, Yang Yunxia and Shouqi Xie*. Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology. Aquaculture Nutrition2017. 23:129-147.
13. Haokun Liu, Quangen Yan, Junyan Jin, Yunxia Yang, Dong Han, Xiaoming Zhu and Shouqi Xie*. Effect of dietary cottonseed meal on growth performance, physiological responses and gossypol accumulation of pre-adult grass carp Ctenopharyngodon idellus. Chinese Journal of Oceanology and Limnology, 2016, 5: 992-1003.
14. 陈林, 朱晓鸣, 韩冬, 杨云霞, 金俊琰, 刘昊昆, 解绶启*。芙蓉鲤鲫幼鱼饲料适宜淀粉含量。水生生物学报,2016,40 (4): 690-699。
15. 曹申平,韩冬,解绶启,金俊琰,刘昊昆,杨云霞,朱晓鸣*。螺旋藻粉替代饲料中鱼粉对异育银鲫幼鱼生长、饲料利用和蛋白沉积的影响。水生生物学报2016,40 (4): 647-654。
16. 单秀娟, 韩冬, 张文兵, 陈宇顺, 王清印, 解绶启, 李钟杰, 张国范, 麦康森, 徐跑, 李家乐, 唐启升, 2015. 中国水产养殖缓解了对野生渔业资源需求的压力, 中国水产, 2015(6):5-6.
17. Haokun Liu, Xiaoming Zhu, Yang Yunxia, Dong Han, Junyan Jinand Shouqi Xie*. Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): nutrient digestibility, growth performance, body composition and morphometry. Aquaculture Nutrition. 2016. 22:142-157.
18. Shuaibing Zhao, DongHan, Xiaoming Zhu, Junyan Jin, Yunxia Yang & Shouqi Xie*. Effects of feeding frequency and dietary protein levels on juvenile allogynogenetic gibel carp (Carassius auratus gibelio) ver. CAS III: growth, feed utilization and serum free essential amino acids dynamics. Aquaculture Research. 2016. 47:290-303.
19. Xiangsong Li,Xiaoming Zhu,Dong Han, Yunxia Yang, Junyan Jin & Shouqi Xie*. Carbohydrate utilization by herbivorous and omnivorous freshwater fish species: a comparative study on gibel carp (Carassius auratus gibelio. var CAS III) and grass carp (Ctenopharyngodon idellus). Aquaculture Research. 2016. 47:128-139.
20. Liangxing Zhou, Dong Han, Xiaoming Zhu, Yunxia Yang, Junyan Jin and Shouqi Xie*. 2016, Effects of total replacement of fish oil by pork lard or rapeseed oil and recovery by a fish oil finishing diet on growth, health and fish quality of gibel carp (Carassius auratus gibelio). Aquaculture Research 47:2961–2975
21. Bingyuan Yang, Cuicui Wang, Huihua Hu, Yongqin Tu, Dong Han*, Xiaoming Zhu, Junyan Jin, Yunxia Yang, Shouqi Xie, 2015. Repeated handling compromises the immune suppression and improves the disease resistance in overwintering channel catfish (Ictalurus punctatus). Fish & Shellfish Immunology 47, 418-428.
22. Bingyuan Yang, Cuicui Wang, Yongqin Tu, Huihua Hu, Dong Han*, Xiaoming Zhu, Junyan Jin, Yunxia Yang, Shouqi Xie, 2015. Effects of repeated handling and air exposure on the immune response and the disease resistance of gibel carp (Carassius auratus gibelio) over winter. Fish & Shellfish Immunology, 47:933-941.
23. Stéphanie Fontagné-Dicharry, Simon Godin, Haokun Liu, Philip Antony Jesu Prabhu, Brice Bouyssière, Ma?té Bueno, Philippe Tacon, Fran?oise Médale and Sadasivam J. Kaushik. 2015. Influence of the form and levels of dietary selenium on antioxidant status and oxidative stress-related parameters in rainbow trout (Oncorhynchus mykiss) fry. British Journal of Nutrition, 113:1876-1887. ( JUN 28 2015, IF 3.45)
24. 李向松,郭志强,韩冬,朱晓鸣,杨云霞*,金俊琰,解绶启。2015. 杂交鲟对六种蛋白原料的表观消化率。水生生物学报,2015,39,432-436。
25. Yongqin Tu, Shouqi Xie, Dong Han, Yunxia Yang, Junyan Jin, Haokun Liu, Xiaoming Zhu*. 2015. Growth performance, digestive enzyme, transaminase and GH-IGF-I axis gene responsiveness to different dietary protein levels in broodstock allogenogynetic gibel carp (Carassius auratus gibelio) CAS III. Aquaculture . 446:290-297.
26. Yongqin Tu, Shouqi Xie, Dong Han, Yunxia Yang, Junyan Jin, Xiaoming Zhu*. 2015. Dietary arginine requirement for gibel carp (Carassis auratus gibelio var. CAS III) reduces with ?sh size from 50 g to 150 g associated with modulation of genes involved in TOR signaling pathway. Aquaculture. 449: 37–47.
27. Wenjuan Ye, Dong Han,Xiaomin Zhu, Yunxia Yang, Junyan Jin, Shouqi Xie*.2015. Comparative studies on dietary protein requirements of juvenile and on-growing gibel carp (Carassius auratus gibelio) based on fishmeal-free diets.Aquaculture Nutrition.21: 286-299.
28. Cuicui Wang, Dong Han, Xiaoming Zhu,Junyan Jin, Yunxia Yang, Shouqi Xie*. 2015. Responses to fishmeal and soybean meal-based diets by three kinds oflarval carps of different food habits.Aquaculture Nutrition. 21:552-568.
29. Quangen Yan , Xiaoming Zhu, Yunxia Yang, Dong Hanand Shouqi Xie* .2015. Partial Replacement of Fishmeal with Alternative Protein Sources in Diets for Korean Rockfish, Sebastes schlegeli. Journal of Ocean University of China. 13(6):1054-1060.
30.Junyan Jin, Francoise Médale, Bijusam Kamalam, Peyo Aguirre, Vicent Véron and Stephane Panserat. 2014. Comparison of glucose and lipid metabolic gene expressions between fat and lean lines of rainbow trout after a glucose load. Plos ONE, 9(8): e105548:1-15.
31. Junyan Jin, Stephane Panserat, Bijusam Kamalam, Peyo Aguirre, Vicent Véron and Francoise Médale. 2014, Insulin regulates lipid and glucose metabolism similarly in two lines of rainbow trout divergently selected for muscle fat content. General ComparativeEndorinology, 204: 49-59.
32. Ying Huang,Dong Han,Xiaoming Zhu*, Yunxia Yang, Junyan Jin, Yifeng Chen, Shouqi Xie. 2014, Effect of dietary aflatoxin B1 on growth, fecundity and tissue accumulation in gibel carp during the stage of gonad development. Aquaculture, 428–429 : 236–242.
33. Jiancheng Zhou, Dong Han, Junyan Jin, Shouqi Xie, Yunxia Yang & Xiaoming Zhu*. 2014, Compared to fish oil alone, a corn and fish oil mixture decreases the lipid requirement of a freshwater fish species, Carassius auratus gibelio. Aquaculture, 428–429: 272–279.
34. Wangbao Gong, Wu Lei, Xiaoming Zhu, Yunxia Yang, Dong Han & Shouqi Xie*. 2014. Dietary myo-inositol requirement for juvenile gibel carp (Carassius auratus gibelio). Aquaculture Nutrition20: 514-519.
35. Yuhang Chen, Xiaoming Zhu, Yunxia Yang, Dong Han, Junyan Jin, Shouqi Xie*.2014. Effect of dietary chitosan on growth performance, hematology, immune response, intestine morphology, intestine microbiota and disease resistance in gibel carp (Carassius auratus gibelio).Aquaculture Nutrition. 20: 532-546.
36. Yuhang Chen, Xiaoming Zhu, Yunxia Yang, Dong Han, Junyan Jin, Shouqi Xie*. 2014.Effect of dietary lysozyme on growth, immune response, intestine microbiota, intestine morphology and resistance toAeromonas hydrophilia in gibel carp (Carassius auratusgibelio).Aquaculture Nutrition,20:229-241.
37. 种香玉, 周立志, 韩冬, 朱晓鸣, 杨云霞, 金俊琰, 解绶启*。2014。饲料中铜浓度对异育银鲫和斑点叉尾鮰的影响。水生生物学报,38(4):751-763。
38. 陈宇航,解绶启*,韩冬,朱晓鸣,杨云霞。2014。 饲料中灵芝葡聚糖提取物对异育银鲫(Carassius auratus gibelio)生长、饲料利用、免疫应答及抗病的影响。水生生物学报,38(4):609-618。
39. Xucheng Xiao, Dong Han, Xiaoming Zhu*, Yunxia Yang , Shouqi Xie, Ying Huang. 2014. Effect of dietary cornstarch levels on growth performance, enzyme activity and hepatopancreas histology of juvenile Red Swamp Crayfish, Procambarus clarkii (Girard).Aquaculture 426-427: 112-119。
40. Guifang Dong, Xiaoming Zhu*, Hongxiang Ren, Boyao Nie, Lu Chen, Hui Li & Bo Yan. 2014. Effects of oxidized fish oil intake on tissue lipidmetabolism and fatty acid composition of channelcatfish (Ictalurus punctatus). Aquaculture Research. 45:1867-1880.
41. 刘晓庆,解绶启,韩冬,朱晓鸣*,杨云霞,金俊琰。2014。饲料鱼粉、菜粕比例对异育银鲫(Carassius auratus gibelio)生长和饲料利用的影响。水生生物学报,38(4):657-663。
42. 叶文娟,朱晓鸣,韩冬,杨云霞,解绶启*。2014。饲料蛋白水平对泥鳅幼鱼生长和饲料利用的影响。水生生物学报,38(3):571-575。
43. 李海燕,解绶启*,韩冬,朱晓鸣,杨云霞,金俊琰。2014。上市前限喂对池塘养殖异育银鲫生长及品质的影响。水生生物学报,38(3):525-532。
44. 严全根,解绶启*,韩冬,朱晓鸣,杨云霞,金俊琰。2014。饲料中棉粕替代鱼粉蛋白对草鱼的生长、血液生理和鱼体组成的影响。水生生物学报,38 (2): 362-369。
45. 段元慧,解绶启*,朱晓鸣,韩冬,杨云霞。2013. 异育银鲫幼鱼对饲料中维生素K需求的研究。水生生物学报,37:8-15。
46. P. Rajeev Raghavan,朱晓鸣,雷武,韩冬,杨云霞,解绶启*。2013. Rearing tank color influences survival and growth of the early larvae of the Chinese Yellow catfish, Pelteobagrus fulvidraco, Richardson,水生生物学报, 37 (2): 177-184。
47. 刘变枝,朱晓鸣,雷武,韩冬,杨云霞,金俊琰,解绶启*。2013. 卤虫投喂下长吻鮠仔稚鱼消化酶发育和口宽变化的研究。水生生物学报,37(1):125-131。
48. 刘变枝,朱晓鸣,韩冬,杨云霞,金俊琰,解绶启*。2013. 投喂水平对长吻鮠仔稚鱼生长和存活的影响。水生生物学报,37 (2): 261-268 。
49. Bianzhi Liu, Xiaoming Zhu, Wu Lei, Yunxia Yang, Dong Han, JunyanJin, Shouqi Xie*. 2012. Effects of different weaning strategies on survival and growth in Chineselongsnout catfish (Leiocassis longirostris Günther) larvae. Aquaculture 364-365:13-18.
50. Yuanhui Duan, Xiaoming Zhu, Dong Han, Yunxia Yang, Shouqi Xie*, 2012. Dietary choline requirement in slight methionine-deficient diet for juvenile gibel carp (Carassius auratus gibelio). Aquaculture Nutrition 18:620-627.
专利:
1. 朱晓鸣,聂光汉,雷武,杨云霞,解绶启。2002. 鱼类呼吸测定装置。ZL 02 2 28280.7。
2. 聂光汉,雷武,解绶启,朱晓鸣,杨云霞。2003. 鱼用自动投饵装置。ZL 02 2 28281.5 。
3. 雷武,聂光汉,解绶启,朱晓鸣,杨云霞。2006. 室内鱼类养殖水分压装置。ZL 2005 2 0095393.5。
4. 雷武,聂光汉,解绶启,朱晓鸣,杨云霞。2006. 室内鱼类养殖分压串联装置。ZL 2005 2 0095394.X。
5. 聂光汉,雷武,解绶启,朱晓鸣,杨云霞。2006. 室内鱼类养殖管道装置。ZL 2005 2 0095395.4。
6. 韩冬,解绶启,朱晓鸣,杨云霞,聂光汉。2013. 一种长吻鮠养殖动态投饲表的建立方法。ZL 2010 1 0100337.1