学科带头人：周炳升

学科组成员：周炳升 博士，研究员

学科组介绍：环境毒理组目前进行的研究方向是持久性有机污染物 (POPs) 对生物作用的分子机制及生态毒理学效应。研究主要在两个方面进行:
(1) 以培养的细胞株或原代细胞培养，用来研究污染物作用的分子机制。建立灵敏、高效的生物毒性测试方法，评价有机化学品的潜在毒性效应。
(2) 研究有毒污染物在水环境中的迁移转化过程及生态风险。以小型淡水鱼作为活体模型研究分子机理及生态学效应。.研究工作将微观与宏观相结合，从分子/生化/生理/个体层次上进行，并建立之间的联系，探索有机污染物对水生生物的生殖毒性、神经毒性、发育毒性、健康危害，以及多种污染物在环境条件下的复合作用，寻找更敏感、有效的生物标记物，发展环境污染的早期预警系统，从而为有机污染物的危险评价以及为我国可持续发展战略、生态安全及环境保护决策提供理论依据。

学科组现得到国家科技部863计划、国家自然科学基金项目、中国科学院知识创新重要方向项目等项目的资助:
1. 典型持久性有机污染物的环境过程与毒理效应：子课题“典型持久性有机污染物的毒理效应”（国家自然科学基金重大项目）
2. 典型全氟代有机化合物的发育毒性及雌激素效应的分子机理研究（国家自然科学基金面上项目）
3. 有毒污染物的分子毒理与筛选技术子课题：离体细胞系的构建和有毒污染物离体筛选技术的研究（中科院知识创新重要方向项目）
4. 溴系阻燃剂暴露及效应的评估新技术（国家863计划）
5. 有机污染物全氟辛烷磺酰基化合物的分子生态毒理学效应及危险评价 （自然科学基金面上项目）
6. 持久性有机污染物的分子生态毒学效应及生物可降解性研究 （中国科学院知识创新重要方向项目）
7. 建立新的生物学模型以筛选和评价有机化合物的毒性及对水生态环境的影响
8. 环境污染物持续性作用的健康效应及致病机制研究（湖北省科技攻关计划）

仪器设备：倒置荧光显微镜，紫外-可见光-荧光酶标仪，超纯水制备系统，细胞培养系列设备，PCR仪，凝胶成像，高速冷冻离心机，超低温冰箱，杂交箱，切片机。

近三年发表文章：
1. Xiongjie Shi, Leo W. Y. Yeung, Paul K. S. Lam, Rudolf S. S. Wu, Bingsheng Zhou* (2009). Protein Profiles in Zebrafish (Danio rerio) Embryos Exposed to PFOS. Toxicological Sciences (in press).
2. Chunsheng Liu, Liqin Yu, Jun Deng, Paul K. S. Lam, Rudolf S. S. Wu, Bingsheng Zhou* (2009). Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic–pituitary–gonadal (HPG) axis of zebrafish. Aquatic Toxicology (in press).
3. Jun Deng, Liqin Yu, Chunsheng Liu, Ke Yu, Xiongjie Shi, Leo WY Yeung, Paul KS Lam, Rudolf SS Wu, Bingsheng Zhou*(2009). HBCD-induced developmental toxicity and apoptosis in zebrafish embryos. Aquatic Toxicology (in press). 
4. 史熊杰，刘春生，余珂，邓军，余丽琴，周炳升*（2009）。环境内分泌干扰物毒理学研究进展与展望。化学进展，21，340-349。
5. Yongbing Du, Xiongjie Shi, Chunsheng Liu, Ke Yu, Bingsheng Zhou* (2009). Chronic effects of water-borne PFOS exposure on growth, survival and hepatotoxicity in zebrafish: A partial life-cycle test. Chemosphere, 74, 723-729.
6. Ke Yu, Yuhe He, Leo W.Y. Yeung, Paul KS Lam, Rudolf SS Wu, Bingsheng Zhou* (2008). DE-71-induced apoptosis involving intracellular calcium and the Bax-mitochondria-caspase protease pathway in human neuroblastoma cells in vitro. Toxicological Sciences, 104, 341-351.
7. Xiongjie Shi, Yongbing Du, Paul KS Lam, Rudolf SS Wu, Bingsheng Zhou* (2008). Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS. Toxicology & Applied Pharmacology, 230, 23-32. 
8. Chunsheng Liu, Yongbing Du, Bingsheng Zhou* (2007). Evaluation of estrogenic activities and mechanism of action of perfluorinated chemicals determined by vitellogenin induction in primary cultured tilapia hepatocytes. Aquatic Toxicology, 85，267-277. 
9. So M.K., Y. Miyake, W.Y. Yeung, Y.M. Ho, S. Taniyasu, P. Rostkowski, N. Yamashita, B.S. Zhou, X.J. Shi, J.X. Wang, J.P. Giesy, H. Yu and P.K.S. Lam* (2007). Perfluorinated compounds in the Pearl River and Yangtze River of China. Chemosphere, 68, 2085-2095.
10. Wen Yi, Wang Ying, Zhou Bing-Sheng, Xu Ying and Feng Yu-Qi* (2007). Determination of Sexual Hormones in Liquid Cosmetics by Polymer Monolith Microextraction Coupled with High Performance Liquid Chromatography. Chinese Journal of analytical Chemistry, 35, 681-684.
11. Chunsheng Liu, Ke Yu, Xiongjie Shi, Jingxian Wang, Paul K.S. Lam, Rudolf S.S. Wu and Bingsheng Zhou* (2007). Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Aquatic Toxicology, 82:135-143.
12. Ling Ding, Margaret B. Murphy, Henry Y.H. He, Yan Xu, Leo W.Y. Yeung, Jingxian Wang, Bingsheng Zhou*, Paul K.S. Lam, Rudolf S.S. Wu and John P. Giesy (2007). Effects of brominated flame retardants and brominated dioxins on steroidgenesis in H295R human adrenocortical carcinoma cell line. Environmental Toxicology & Chemistry, 26: 764-772.
13. Yi Wen, Bingsheng Zhou, Ying Xu, Shiwei Jin, Yuqi Feng* (2006). Analysis of estrogens in environmental waters using polymer monolith in polyether ether ketone tube solid-phase microextraction combined with high-performance liquid chromatograph. Journal of Chromatography A, 1133: 21-28. 
14. S.B. Song, Y. Xu* and  B.S. Zhou (2006). Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio). Chemosphere, 65: 699-706.
15. Bingsheng Zhou, Wenhua Liu, William H.L. Siu, Paul K.S. Lam and Rudolf S.S. Wu* (2006). Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress. Aquatic Toxicology, 77: 136-142.
16. Bingsheng Zhou*, Chunsheng Liu, Jingxian Wang, Paul K.S. Lam, Rudolf S.S. Wu (2006). Primary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia. Aquatic Toxicology, 80, 109-118.