研究组组长：缪炜 miaowei@ihb.ac.cn 

研究组成员：

熊杰 (研究员) 、龚迎春 (研究员) 

黄洁 (副研究员)、罗晓甜 (副研究员) 、姜传奇 (副研究员)

袁冬霞 (实验师)、王光营（特别研究助理）、张晶（特别研究助理）、陈凯（特别研究助理）、程子昕（特别研究助理）、谷思雨（特别研究助理）

研究生16名，其中博士研究生6名，硕士研究生10名

学科组介绍：

传承于享誉国内外的原生动物学家王家楫院士创建、沈韫芬院士发展的原生动物分类与生态学学科组。以单细胞真核微生物——原生动物为研究对象，综合利用形态学、分子生物学、组学、生物信息学和实验生物学等多种手段，研究原生动物的分类和生物系统学，建立关键种质和遗传资源；解析原生动物的重要性状和功能演化及生态适应机制；发展原生动物合成生物学模型和关键生物技术，开展原生动物资源利用和应用研究。 　  

近期主要研究方向： 

1）原生动物多样性研究：

长期广泛开展野外调查，发展环境DNA和单细胞组学等关键技术，对原生动物主要类群和自由生/寄生/共生代表种类进行收集、鉴定和分类，建立原生动物种质、遗传资源信息库以及环境DNA数据库；结合形态、组学及基因功能等多维数据，理解原生动物系统发育关系，生物多样性及其形成机制；发展纤毛虫、鞭毛虫、变形虫等多种原生动物研究模型，研究单细胞原生动物适应性进化/生态适应的功能基础，深入探讨生殖、运动、捕食和共生等重要基础或进化生物学问题。 

2）原生动物生物技术：

发掘纤毛虫、鞭毛虫、变形虫原生动物中重要种质和遗传资源，发展以四膜虫为主的单细胞原生动物合成生物学模型，建立基因编辑、大片段DNA合成和组装、细胞赋能等关键技术，研发用于环境污染物监测/降解、有害藻类控制和农业病害防治的新技术和新策略。　  

代表性论文与著作: 

    1.Yang Ma^#, Guanxiong Yan^#, Jing Zhang, Jie Xiong, Wei Miao*. Cip1, a CDK regulator determines heterothallic mating or homothallic selfing in a protist. PNAS, 2024, 121 (13): e2315531121.

    2.Chuanqi Jiang, Siyu Gu, Tingting Pan, Xueyan Wang, Weiwei Qin, Guangying Wang, Xinxin Gao, Jing Zhang, Kai Chen, Alan Warren, Jie Xiong, Wei Miao*. Dynamics and timing of diversification events of ciliated eukaryotes from a large phylogenomic perspective. Molecular Phylogenetics and Evolution, 2024, 197, 108110.

    3.Mingyue Cheng^#, Shuai Luo^#, Peng Zhang^#, Guangzhou Xiong, Kai Chen, Chuanqi Jiang, Fangdian Yang, Hanhui Huang, Pengshuo Yang, Guanxi Liu, Yuhao Zhang, Sang Ba, Ping Yin, Jie Xiong*, Wei Miao*, Kang Ning*. A comprehensive genome and gene catalog of aquatic microbiome in Tibetan Plateau. Nature Communications, 2024, 15, 1438.

    4.Xinxin Gao^#, Kai Chen^#, Jie Xiong^#, Dong Zou^#, Fangdian Yang, Yingke Ma, Chuanqi Jiang, Xiaoxuan Gao, Guangying Wang, Siyu Gu, Peng Zhang, Shuai Luo, Kaiyao Huang, Yiming Bao, Zhang Zhang*, Lina Ma*, Wei Miao*. The P10K database: A data portal for the protist 10,000 genomes project. Nucleic Acids Research, 2024, 52(D1): D747-D755.

    5.Chuanqi Jiang^#, Guangying Wang^#, Jing Zhang, Siyu Gu, Xueyan Wang, Weiwei Qin, Kai Chen, Dongxia Yuan, Xiaocui Chai, Mingkun Yang, Fang Zhou, Jie Xiong*, Wei Miao*. iGDP: an integrated Genome Decontamination Pipeline for wild ciliated microeukaryotes. Molecular Ecology Resources, 2023, 23(5): 1182-1193.

    6.Jing Zhang^#, Weiwei Qin ^#, Che Hu^#, Siyu Gu^#, Xiaocui Chai, Mingkun Yang, Fang Zhou, Xueyan Wang, Kai Chen, Guanxiong Yan, Guangying Wang, Chuanqi Jiang, Alan Warren, Jie Xiong*, Wei Miao*. Giant proteins in a giant cell: molecular basis of ultrafast Ca2+-dependent cell contraction. Science Advances, 2023, 9(8): eadd6550.

    7.Mingyang Ma, Chaojun Wei, Wenjie Huang, Yue He, Yingchun Gong*, Qiang Hu*. A systematic review of the predatory contaminant Poterioochromonas in microalgal culture. Journal of Applied Phycology, 2023, 35, 1103-1114.

    8.Wenya Song, Jingyi Dong, Xiaoteng Lu, Saleh A Al-Farraj, Weibo Song, Hunter N Hines, Xiaotian Luo*. Morphological, ontogenetic, and molecular investigations of freshwater hypotrich ciliates from China revealed a new genus Heterodeviata gen. nov. (Protista: Ciliophora), and a novel limnetic population of Deviata multilineae. Zoological Journal of the Linnean Society, 2023, 199, 263-279.

    9.Chengzhi Mao, Xiao Wang, Xinghao Li, Qiaoyi Kong, Elvis Genbo Xu*, Jie Huang*. Microbial communities, resistance genes, and resistome risks in urban lakes of different trophic states: internal links and external influences. Journal of Hazardous Materials Advances, 2023, 9, 100233.

    10.Chengzhi Mao#, Qi Li^#, Majid Komijani, Jie Huang*, Tao Li*. Metagenomic analysis reveals the dissemination mechanisms and risks of resistance genes in plateau lakes. iScience, 2023, 26, 107508.

    11.Jiawei Tu, Tian Li, Zihan Gao, Jie Xiong, Wei Miao*. Construction of CdS-Tetrahymena thermophila hybrid system by efficient cadmium adsorption for dye removal under light irradiation. Journal of Hazardous Materials, 2022, 439, 129683.

    12.Hanwen Zhang, David J Patterson, Yue He, Hongxia Wang, Danni Yuan, Qiang Hu, Yingchun Gong*. Kinopus chlorellivorus gen. nov., sp. nov. (Vampyrellida, Rhizaria), a new algivorous protist predator isolated from large-scale outdoor cultures of Chlorella sorokiniana. Applied and Environmental Microbiology, 2022, 88(22): e0121522.

    13. 姜传奇, 熊杰, 黄洁, 冯伟松, 龚迎春, 缪炜. 微型生物群落 eDNA-PFU 监测.水生生物学报, 2021 Nov 1;45(6).

    14.Mingyang Ma^#, Fuchen Wang^#, Chaojun Wei, Jianping Chen, Hu Jin,  Hongxia Wang, Lirong Song, Qiang Hu, Yingchun Gong*. Establishment of high-cell-density heterotrophic cultivation of Poterioochromonas malhamensis contributes to achieving biological control of Microcystis. Journal of Applied Phycology, 2022, 34, 423-434.

    15.Pubo Chen^#, Jie Huang^#, Liuyu Rao, Wengen Zhu, Yuhe Yu, Fanshu Xiao*, Huang Yu, Yongjie Wu, Ruiwen Hu, Xingyu Liu, Zhili He, Qingyun Yan*. Environmental effects of nanoparticles on the ecological succession of gut microbiota across zebrafish development. 2022, Science of the Total Environment, 806, 150963.

    16.Xiaotian Luo, William A Bourland, Weibo Song, Jie Huang*. New contributions to the taxonomy of urostylid ciliates (Ciliophora, Hypotrichia), with establishment of a new genus and new species. European Journal of Protistology, 2021, 80, 125810.

    17.Fanshu Xiao^#, Wengen Zhu^#, Yuhe Yu, Zhili He, Bo Wu, Cheng Wang, Longfei Shu, Xinghao Li, Huaqun Yin, Jianjun Wang, Philippe Juneau, Xiafei Zheng, Yongjie Wu, Juan Li, Xiaojuan Chen, Dongwei Hou, Zhijian Huang, Jianguo He, Guohuan Xu, Liwei Xie, Jie Huang*, Qingyun Yan*. Host development overwhelms environmental dispersal in governing the ecological succession of zebrafish gut microbiota. npj Biofilms and Microbiomes, 2021, 7, 5.

    18.Jie Xiong, Wentao Yang, Kai Chen, Chuanqi Jiang, Yang Ma, Xiaocui Chai, Guanxiong Yan, Guangying Wang, Dongxia Yuan, Yifan Liu, Shelby L. Bidwell, Nikhat Zafar, Michalis Hadjithomas, Vivek Krishnakumar, Robert S. Coyne, Eduardo Orias, Wei Miao*. Hidden genomic evolution in a morphospecies—The landscape of rapidly evolving genes in Tetrahymena. PLOS Biology, 2019, 17(6): e3000294.

    19.Xinghao Li, Jie Huang*, Sabine Filker, Thorsten Stoeck, Yonghong Bi*, Yuhe Yu, Weibo Song. Spatio-temporal patterns of zooplankton in a main-stem dam affected tributary: a case study in the Xiangxi River of the Three Gorges Reservoir, China. Science China-Life Sciences, 2019, 62, 1058-1069.

    20.Miao Tian, Wentao Yang, Jing Zhang, Huai Dang, Xingyi Lu, Chengjie Fu, Wei Miao*. Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease. Nucleic Acids Research, 2017, 45(11): 6848-6863.

    21.Jie Xiong, Shan Gao, Wen Dui, Wentao Yang, Xiao Chen, Sean D. Taverna, Ronald E. Pearlman, Wendy Ashlock, Wei Miao* and Yifan Liu*. Dissecting relative contributions of cis- and trans-determinants to nucleosome distribution by comparing Tetrahymena macronuclear and micronuclear chromatin. Nucleic Acids Research, 2016, 44(21):10091-10105.

    22.韦薇, 袁冬霞, 杨文涛, 张晶, 陈瑛*, 缪炜*. 原生动物柯立斯四膜虫(Tetrahymena corlissi)超高砷耐受性的分子机制研究. 中国科学:生命科学, 2016, 46(03): 285-293.

    23.Marcella D Cervantes , Eileen P Hamilton, Jie Xiong, Michael J Lawson, Dongxia Yuan, Michalis Hadjithomas, Wei Miao*, Eduardo Orias*. Selecting one of several mating types through gene segment joining and deletion in Tetrahymena thermophila. PLOS Biology, 2013, 11(3): e1001518.

    24.Jie Xiong, Yuming Lu, Jinmei Feng, Dongxia Yuan, Miao Tian, Yue Chang, Chengjie Fu, Guangying Wang, Honghui Zeng*, Wei Miao*. Tetrahymena functional genomics database (TetraFGD): an integrated resource for Tetrahymena functional genomics. Database, 2013, 2013, bat008.