水生植物生理学研究组
研究组成员： 侯宏伟 研究员；houhw@ihb.ac.cn；
杨晶晶 副研究员,电子信箱：yangjj@ihb.ac.cn；
李高洁 助理研究员,电子信箱：ligaojie@ihb.ac.cn;
李晓哲 助理实验师,电子信箱：lixiaozhe@ihb.ac.cn；
赵旭耀 特别研究助理,电子信箱：zhaoxuyao@ihb.ac.cn；
本研究组从事大型水生植物的研究与应用。
1、水生植物生态、进化与发育生物学研究。自然界生态因子的变化如何影响植物发育过程？植物发育过程如何响应生态因子的变化？“植物-环境”相互作用与物种的适应性进化和生物多样性之间存在紧密的联系，物种的可塑性发育途径对其生态适应和进化模式具有深远的影响。水生植物为适应环境温度、光照、水位等变化，不仅演化出适应不同水陆生境的特征，还演化出了特有的水陆生境表型可塑性，本研究组在前期的探索中，发现一种爵床科的水族植物-异叶水蓑衣（Hygrophilla difformis）个体适中，异形叶特性明显，是研究植物与环境的相互作用的理想材料（图1），进而从形态、生理、细胞和分子等方面研究其生物学特性及其对关键环境因子（温度、光照、CO2、营养等）的应答策略，建立了研究模型，得以重新认识和评价自然界生物遗传和进化的机制。此外，我们还研究浮萍、苦草、黄花狸藻等不同类群的水生植物，解析其淡水生态系统中水生植物的生物多样性及其生态学意义（图2）。
2、浮萍研究与应用。浮萍是高等植物中一类个体小且简化、生长快速的水生植物，是研究植物生理生化和生态毒理的理想材料，又由于其蛋白等含量高、营养全面等特性，在开发替代蛋白食品、饲料和生物质能等方面有巨大开发潜力（图3）。本研究组依托国家水生生物资源库，致力于浮萍种质资源的收集和保藏，在此基础上，开展不同浮萍的生物学特性、营养和基因组研究和比较，挖掘功能基因，解析氮磷代谢和光合作用机制，建立遗传转化和高效培养的方法，创制高光合、高产、高蛋白浮萍种质，优化生产工艺，为建设浮萍垂直生产工厂、开发浮萍替代蛋白提供理论和技术支撑。
3、水生植物在生态、环境和生产上的应用。本研究组对梁子湖等长江流域的重要淡水湖泊开展水生植物调查，研究水生植物的分布、多样性演变规律及其在淡水生态系统中的生态功能。筛选重要的水生经济植物品种（水芹等）进行环境修复和生产应用，建立利用水生经济植物联合处理技术净化沼液的循环利用模式（图4），以及生态、高效的池塘鱼-菜综合种养及高效利用模式，并进行示范推广和应用。
研究组代表论文：
1. 杨晶晶,赵旭耀,李高洁,胡诗琦,陈艳,孙作亮，侯宏伟*. 浮萍的研究及应用进展,科学通报. 2021
2. 李高洁,胡诗琦,杨晶晶,侯宏伟*. 植物异形叶研究进展. 植物生理学报,2020
3. 吴志刚,熊文,侯宏伟*. 长江流域水生植物多样性格局与保护. 水生生物学报,2018
4. Li GJ;Zhao XY;Yang JJ;Hu SQ;Ponnu J;Kimura S;Hwang I;Torii KU; Hou HW. Water wisteria genome reveals environmental adaptation and heterophylly regulation in amphibious plants. Plant Cell Environ. 2024
5. Xia ML;Li XZ;Yang JJ;Li GJ;Zhao XY;Hou HW*. Cress-loach coculture for improving the utilization efficiency of biogas slurry in aquaponic systems. Environ Technol & Inno，2023
6. Li YX;Xia ML;Zhao XY;Hou HW*. Water temperature and chlorophyll a density drive the genetic and epigenetic variation of Vallisneria natans across a subtropical freshwater lake. Ecol Evol. 2023.
7. Li YX;Zhao XY;Xia ML;Wei XZ;Hou HW*. Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake,Plant Diversity.2023.
8. Sun ZL; Zhao XY; Li GJ; Yang JJ; Chen Y; Xia ML; Hwang I; Hou HW*, Metabolic flexibility during a trophic transition reveals the phenotypic plasticity of greater duckweed (Spirodela polyrhiza 7498), New Phytol. 2023,
9. Li FI; Yang JJ; Sun ZY； Wang L； Qi LY；A S； Liu YQ； Zhang HM； Dang LF； Wang SJ； Luo CX； Nian WF； O'Conner S； Ju LZ； Quan WP； Li XK； Wang C； Wang DP； You HL； Cheng ZK； Yan J； Tang FC； Yang DC； Xia CW； Gao G； Wang Y； Zhang BC； Zhou YH； Guo X； Xiang SH； Liu H； Peng TB； Su XD； Chen Y； Ouyang Q； Wang DH； Zhang DM； Xu ZH； Hou HW； Bai SN； Li L. Plant-on-chip: Core morphogenesis processes in the tiny plant Wolffia australiana. PNAS Nexus. 2023
10. Ikematsu S； Umase T; Shiozaki M; Nakayama S; Noguchi F; Sakamoto T; Hou HW; Gohari G; Kimura S; Torii KU. Rewiring of hormones and light response pathways underlies the inhibition of stomatal development in an amphibious plant Rorippa aquatica underwater. Curr Biol. 2023
11. Li GJ; Yang, JJ; Chen YM; Zhao, XY; Chen Y; Kimura S; Hu SQ; Hou,HW*, SHOOT MERISTEMLESS participates in the heterophylly of Hygrophila difformis (Acanthaceae). Plant Physiol, 2022
12. Sun ZL; Zhao XY; Xia ML; Yang JJ; Chen Y; Li XZ; Hou,HW*, Use of hemicellulose-derived xylose for environmentally sustainable starch production by mixotrophic duckweed. Sustain Energ Fuels, 2022
13. Zhao XY; Yang JJ; Li XZ; Li GJ; Sun ZL; Chen Y; Chen YM; Xia ML; Li YX; Yao LG; Hou HW*, Identification and expression analysis of GARP superfamily genes in response to nitrogen and phosphorus stress in Spirodela polyrhiza. BMC Plant Biol, 2022
14. Sun ZL; Guo WJ; Zhao XY; Chen Y; Yang JJ; Xu SQ; Hou HW*, Sulfur limitation boosts more starch accumulation than nitrogen or phosphorus limitation in duckweed (Spirodela polyrhiza). Ind Crop Prod, 2022
15. Yang JJ; Li GJ; Xia ML; Chen YM; Chen Y; Kumar S; Sun ZL; Li XZ; Zhao XY*; Hou HW*, Combined effects of temperature and nutrients on the toxicity of cadmium in duckweed (Lemna aequinoctialis). J Hazard Mater, 2022
16. Yang JJ; Zhao XY; Chen Y; Li GJ; Li XZ; Xia ML; Sun ZL; Chen YM; Li YX; Yao LG; Hou HW*, Identification, structural, and expression analyses of SPX genes in giant duckweed (Spirodela polyrhiza) reveals its role in response to low phosphorus and nitrogen Stresses. Cells, 2022
17. Chen Y; Li GJ; Yang JJ; Zhao XY; Sun ZL; Hou HW*, Role of nramp transporter genes of Spirodela polyrhiza in cadmium accumulation. Ecotox Environ Safe, 2021
18. Yang JJ; Zhao XY; Li GJ; Hu SQ; Hou HW*, Frond architecture of the rootless duckweed Wolffia globosa. BMC Plant Biol, 2021
19. Kumar S; Li GJ; Yang JJ; Huang XF; Ji Q; Liu ZW; Ke WD*; Hou HW*, Effect of salt stress on growth, physiological parameters, and ionic concentration of water dropwort (Oenanthe javanica) cultivars. Front Plant Sci. 2021
20. Zhao XY; Li GJ; Sun ZL; Chen Y; Guo WJ; Li YX; Chen YM; Yang JJ*; Hou HW*, Identification, structure analysis, and transcript profiling of phosphate transporters under Pi deficiency in duckweeds. Int J Biol Macromol. 2021
21. Zhao XY; Yang JJ; Li GJ; Sun ZL; Hu SQ; Chen Y; Guo WJ; Hou HW*, Genome-wide identification and comparative analysis of the WRKY gene family in aquatic plants and their response to abiotic stresses in giant duckweed (Spirodela polyrhiza). Genomics. 2021
22. Kumar S; Li, GJ; Yang JJ; Huang XF; Ji Q; Zhou K; Khan S; Ke WD*; Hou HW*, Investigation of an antioxidative system for salinity tolerance in Oenanthe javanica. Antioxidants. 2020
23. Sun ZL; Guo WJ; Yang JJ; Zhao XY; Chen Y; Yao LG; Hou HW*. Enhanced biomass production and pollutant removal by duckweed in mixotrophic conditions. Bioresour Technol. 2020
24. Heenatigala PPM; Sun ZL; Yang JJ; Zhao XY; Hou HW*, Expression of LamB vaccine antigen in Wolffia globosa (Duckweed) against fish vibriosis. Front Immunol. 2020
25. Li GJ #; Hu SQ #; Yang JJ; Zhao XY; Schultz E; Kimura S; Hou HW*, Establishment of an Agrobacterium mediated transformation protocol for the detection of cytokinin in the heterophyllous plant Hygrophila difformis (Acanthaceae), Plant Cell Rep. 2020
26. Wu ZG; Xu X; Zhang J; Wiegleb G; Hou HW*, Influence of environmental factors on the genetic variation of the aquatic macrophyte Ranunculus subrigidus on the Qinghai-Tibetan Plateau. BMC Evol Biol. 2019
27. Yang JJ; Li GJ; Hu SQ; Bishopp A; Heenatigala PPM; Kumar S; Duan PF; Yao LG; Hou HW*, A protocol for efficient callus induction and stable transformation of Spirodela polyrhiza (L.) Schleiden using Agrobacterium tumefaciens. Aquat Bot. 2018
28. Mariyamma NP; Hou HW; Carland FM; Nelson T; Schultz EA*, Localization of Arabidopsis FORKED1 to a RABA positive compartment suggests a role in secretion. J Exp Bot. 2017
29. Mariyamma N; Clarke K; Yu H; Hou HW; Wilton E; Dyk J; Schultz E*, Members of the Arabidopsis FORKED1-LIKE gene family act to localize PIN1 in developing veins. J Exp Bot. 2018
30. Li GJ; Hu SQ; Yang JJ; Schultz E; Clarke K; Hou HW*, Water-Wisteria as an ideal plant for heterophylly study in higher aquatic plants. Plant Cell Rep. 2017
31. Wu Z; Wu J; Wang Y; Hou HW*, Development of EST-derived microsatellite markers in the aquatic macrophyte Ranunculus bungei (Ranunculaceae). Applications in Plant Sciences. 2017
32. Mariyamma N; Hou HW; Carland F; Nelson T; Schultz E*, Localization of Arabidopsis FORKED1 to a RABA positive compartment suggests a role in secretion. J Exp Bot. 2017
33. Khan S; Nabi G; Ullah MW; Yousaf M; Manan S; Siddique R; Hou HW*, Overview on the role of advance genomics in conservation biology of endangered species. Int J Genomics. 2016
34. Held M#; Hou HW#; Miri M#; Huynh C; Ross L; Hossain S; Sato S; Tabata S; Perry J; Wang T; Szczyglowski K*, Lotus japonicus cytokinin receptors work partially redundantly to mediate nodule formation. Plant Cell. 2014
35. Yoon H#; Hossain M#; Held M; Hou HW; Kehl M; Tromas A; Sato S; Tabata T; Andersen S; Stougaard J; Ross L; Szczyglowski K*, Lotus japonicus SUNERGOS1 encodes apredicted subunit A of a DNA topoisomerase VI and is required for nodule differentiationand accommodation of rhizobial infection. Plant J. 2014
36. Garrett J#; Meents M; Blackshaw M; Blackshaw L; Hou HW; Styranko D; Kohalmi S; Schultz E*, A novel, semi-dominant allele of MONOPTEROS provides insight into leaf initiation and vein pattern formation. Planta. 2012
37. Hou HW; Erickson J; Meservy J; Schultz E*, FORKED1 encodes a PH domain proteinthat is required for PIN1 canalization in developing leaf veins. Plant J. 2010
38. Hou HW; Zhou Y; He X; Mwange K; Li W; Cui K*, The ABP1 expression regulated by IAA and ABA is associated with the cambial activity periodicity in Eucommia ulmoides. J Exp Bot. 2006
39. Mwange, K#; Hou, HW#;Wang, Y; He, X; Cui, K*, Opposite patterns in the annual distribution and time course of endogenous abscisic acid and indole-3-acetic acid in relation to the periodicity of cambial activity in Eucommia ulmoides. J Exp Bot. 2005
40. Mwange, K; Hou, HW; Cui, K*, Relationship between endogenous indole-3-acetic acid and abscisic acid changes and bark recovery in Eucommia ulmoides after girdling. J Exp Bot. 2003
