姓 名: | 孙永华 |
---|---|
性 别: | 男 |
职 称: | 研究员 |
电 话: | 86-27-68780235 |
电子邮件: | yhsun@ihb.ac.cn |
简历介绍:
孙永华,1976年生,博士,中国科学院水生生物研究所研究员,博士生导师,鱼类发育与生物技术学科组组长,鱼类生物学及渔业生物技术研究中心副主任,国家水生生物种质资源库主任,国家斑马鱼资源中心主任。1997年于武汉大学生命科学学院获理学学士学位;2002年于中国科学院研究生院获理学博士学位;2006年9月-2008年3月获法中科学及应用基金会(FFCSA)奖励在法国国家科研中心IGBMC从事博士后研究;2009年10-12 月以Visiting Associate Professor身份在美国弗吉尼亚大学进行合作研究。
主要从事鱼类生殖细胞发育与定向育种研究。揭示调控鱼类卵质决定和早期胚胎发育的关键因子,并阐明其作用的分子机制;建立高效特异的鱼类生殖细胞操作技术及基因编辑配子的“借腹怀胎”技术;牵头完成“斑马鱼1号染色体全基因敲除计划”,主持建成国际学术界公认的全球三大斑马鱼资源库之一——国家斑马资源中心(http://zfish.cn)。先后主持国家自然科学基金优秀青年科学基金(2012)、国家杰出青年科学基金(2020)、国家重点研发计划“发育编程及其代谢调节”重点专项(2019)等一批科研项目。在Genome Research、 Nature Communications、PLOS Biology、PLOS Genetics、Endocrinology以及Science Bulletin、Science China-Life Sciences等国内外学术刊物上发表论文90 余篇。相关论文入选Springer Nature年度亮点论文,被Faculty of 1000专门推荐等。
研究方向:
鱼类生殖细胞发育与定向育种
社会任职:
中国动物学会理事
中国动物学会斑马鱼分会副理事长兼秘书长
获奖及荣誉:
2019:中国科学院水生生物研究所,重要贡献奖
2016:中国科学院青年创新促进会,优秀会员
2016:首届中国水产青年科技奖
2010:中国科学院卢嘉锡青年人才奖
代表论著:
1) He, M., Zhang, R., Jiao, S., Zhang, F., Ye, D., Wang, H., and Sun, Y.* (2020). Nanog safeguards early embryogenesis against global activation of maternal β-catenin activity by interfering with TCF factors. PLOS Biology 18, e3000561.
2) Zhang, Q., Ye, D., Wang, H., Wang, Y., Hu, W., and Sun, Y.* (2020). Zebrafish cyp11c1 Knockout Reveals the Roles of 11-ketotestosterone and Cortisol in Sexual Development and Reproduction. Endocrinology 161, DOI:10.1210/endocr/bqaa048
3) Zhang, F., Li, X., He, M., Ye, D., Xiong, F., Amin, G., Zhu, Z., and Sun, Y.* (2020). Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells. Journal of genetics and genomics 47, 37-47. (Recommended by Faculty of 1000)
4) Sun, Y.*, Zhang, B., Luo, L., Shi, D.L., Wang, H., Cui, Z., Huang, H., Cao, Y., Shu, X., Zhang, W., et al. (2020). Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9. Genome Research 30, 118–126.
5) Li, X., Zhang, F., Wu, N., Ye, D., Wang, Y., Zhang, X., Sun, Y.*, and Zhang, Y.-A*. (2020). A critical role of foxp3a-positive Treg cells in maintaining immune homeostasis in zebrafish testis development. Journal of Genetics and Genomics. DOI: 10.1016/j.jgg.2020.07.006
6) Liu, C., Ye, D., Wang, H., He, M., and Sun, Y.* (2020). Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study. Mar Biotechnol (NY) 22, 613-619.
7) Ye, D., Wang, X., Wei, C., He, M., Wang, H., Wang, Y., Zhu, Z., and Sun, Y.* (2019). Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLOS Genetics 15, e1008306.
8) Sun, Y.*, and Zhu, Z. (2019). Designing future farmed fishes using genome editing. Science China Life sciences 62, 420-422.
9) Ye, D., Zhu, L., Zhang, Q., Xiong, F., Wang, H., Wang, X., He, M., Zhu, Z., and Sun, Y.* (2019). Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline. Mar Biotechnol (NY) 21, 217-228. (Springer Nature 2019 Highlights)
10) Zhang, X., Pang, S., Liu, C., Wang, H., Ye, D., Zhu, Z., and Sun, Y.* (2019). A Novel Dietary Source of EPA and DHA: Metabolic Engineering of an Important Freshwater Species-Common Carp by fat1-Transgenesis. Mar Biotechnol (NY) 21, 171-185.
11) Liu, Y., Xue, W., Zhu, L., Ye, D., Zhu, X., Wang, H., Sun, Y.*, and Deng, F.* (2017). Nanog suppresses the expression of vasa by directly regulating nlk1 in the early zebrafish embryo. Biochimie 142, 93-101.
12) Sun, Y.-H.* (2017). Genome editing opens a new era for physiological study and directional breeding of fishes. Science Bulletin 62, 157-158.
13) Liu, L., Pan, L., Li, K., Zhang, Y., Zhu, Z., and Sun, Y.* (2016). Zebrafish Health Conditions in the China Zebrafish Resource Center and 20 Major Chinese Zebrafish Laboratories. Zebrafish 13 Suppl 1, S8-S18.
14) He, M.-D., Zhang, F.-H., Wang, H.-L., Wang, H.-P., Zhu, Z.-Y., and Sun, Y.-H.* (2015). Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos. Mutation Research 780, 86-96.
15) Xie, X., Pan, L., and Sun, Y.* (2015). Growing with the world: rapid development of the zebrafish research in China and the China Zebrafish Resource Center. Science China Life sciences 58, 396-399.
16) Pang, S.C., Wang, H.P., Zhu, Z.Y., and Sun, Y.H.* (2015). Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish. Mar Biotechnol (NY) 17, 593-603.
17) Ye, D., Zhu, Z., and Sun, Y.* (2015). Fish genome manipulation and directional breeding. Science China Life sciences 58, 170-177.
18) Lu, F.I.#, Sun, Y.H.#, Wei, C.Y., Thisse, C., and Thisse, B. (2014). Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/beta-catenin pathway. Nature Communications 5, 5368. (# co-first author)
19) Pang, S.C., Wang, H.P., Li, K.Y., Zhu, Z.Y., Kang, J.X., and Sun, Y.H.* (2014). Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model. Mar Biotechnol (NY) 16, 580-593.
20) Sun, Y.H.*, and Zhu, Z.Y. (2014). Cross-species cloning: influence of cytoplasmic factors on development. Journal of physiology (Lodon) 592, 2375-2379.
21) Wei, C.Y., Wang, H.P., Zhu, Z.Y., and Sun, Y.H.* (2014). Transcriptional factors Smad1 and Smad9 act redundantly to mediate zebrafish ventral specification downstream of Smad5. The Journal of biological chemistry 289, 6604-6618.
22) Xiong, F., Wei, Z.Q., Zhu, Z.Y., and Sun, Y.H.* (2013). Targeted Expression in Zebrafish Primordial Germ Cells by Cre/loxP and Gal4/UAS Systems. Mar Biotechnol (NY) 15, 526-539.
23) Wang, Y.W., Wei, C.Y., Dai, H.P., Zhu, Z.Y., and Sun, Y.H.* (2013). Subtractive phage display technology identifies zebrafish marcksb that is required for gastrulation. Gene 521, 69-77.
24) Liu, C.W., Xiong, F., Jia, H.Z., Wang, X.L., Cheng, H., Sun, Y.H.*, Zhang, X.Z., Zhuo, R.X., and Feng, J.* (2013). Graphene-based anticancer nanosystem and its biosafety evaluation using a zebrafish model. Biomacromolecules 14, 358-366.
25) Ishtiaq Ahmed, A. S., Xiong, F., Pang, S. C., He, M. D., Waters, M. J., Zhu, Z. Y. and Sun, Y. H.* (2011). Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct. Transgenic Res 20, 557-567.
26) Chen, C.-H., Sun, Y.-H.*, Pei, D.-S. and Zhu, Z.-Y. (2009). Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements. Developmental Dynamics 238, 2850-2859.
27) Sun, Y. H., Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2005). Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (Cyprinus carpio) nuclei and goldfish (Carassius auratus) enucleated eggs. Biology of Reproduction 72, 510-515.
28) Wu, B.#, Sun, Y. H.#, Wang, Y. W., Wang, Y. P. and Zhu, Z. Y. (2005). Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.). Cell Research 15, 447-454.
29) Wu, G.#, Sun, Y. H.# and Zhu, Z. Y. (2003). Growth hormone gene transfer in common carp. Aquatic Living Resources 16, 416-420.
30) Sun, Y., Chen, S., Wang, Y. and Zhu, Z. (2000). The onset of foreign gene transcription in nuclear-transferred embryos of fish. Science China Life Sciences 43, 597-605.
31) Zhu, Z. Y. and Sun, Y. H. (2000). Embryonic and genetic manipulation in fish. Cell Research 10, 17-27.
32) 黄思雨, 何牡丹, 王厚鹏, 孙永华* (2019). 斑马鱼ints12的CRISPR/Cas9敲除及其对UsnRNA剪接的调控. 水生生物学报 43, 1-8.
33) 张峰华, 王厚鹏, 黄思雨, 熊凤, 朱作言, 孙永华* (2016). 两种密码子优化的 Cas9 编码基因在斑马鱼胚胎中基因敲除效率的比较. 遗传 38, 144-154.
34) Wei, Z.-Q., Xiong, F., He, M.-D., Wang, H.-P., Zhu, Z.-Y., and Sun, Y.-H.* (2015). Suppression of Ligase 4 or Xrcc6 activities enhances the DNA homologous recombination efficiency in zebrafish primordial germ cells. Acta Hydrobiol Sin (水生生物学报英文版) 39, 339-348.
35) 王华林, 朱作言, 孙永华* (2015). 斑马鱼socs2的TALEN敲除及其敲除胚胎的生长研究. 水生生物学报 39, 831-836.
36) 于力群, 王厚鹏, 朱作言, 孙永华* (2014). 生长激素/催乳素家族配体和受体成员在斑马鱼早期胚胎中的表达比较和交叉活性分析. 水生生物学报, 809-818.
37) 杨明宇, 王厚鹏, 朱作言, 孙永华* (2014). 斑马鱼ca15b的克隆及在原始生殖细胞中的特异表达. 水生生物学报 38, 142-149.
38) 李阔宇,潘鲁湲,孙永华* (2020). 斑马鱼鱼房和养殖系统建设标准. 中国比较医学杂志 30, 121-127
39) 熊凤, 谢训卫, 潘鲁湲, 李阔宇, 柳力月, 张昀, 李玲璐, 孙永华* (2018). 国家斑马鱼资源中心的资源, 技术和服务建设. 遗传 40, 683-692.
40) 李阔宇, 潘鲁湲, 孙永华* (2014). 斑马鱼资源的开发保藏与国家斑马鱼资源中心. 中国实验动物学报, 93-98,105.
发明专利目录:
1) 熊凤,孙永华,叶鼎,朱作言. 一种用于原始生殖细胞靶向突变的转基因载体及制备方法和用途. 2019年中国发明专利授权,专利号:ZL 201510809203.X
2) 孙永华,庞少臣,康景轩,朱作言.一种提高鱼类omega-3多不饱和脂肪酸的方法及应用. 2016年中国发明专利授权,专利号:ZL 201410155088.4
3) 孙永华,熊凤,魏志强,朱作言. 一种定向筛选同源重组PGC的载体及构建方法和应用. 2015年中国发明专利授权,专利号:ZL 201310341471.4
4) 孙永华, 朱作言, 熊凤, Ishtiaq Ahmed, Mike Waters. 一种鱼类持续激活生长激素受体基因及制备方法和用途. 2013年中国发明专利授权,专利号:ZL 201110141084.7.