| 论文标题： |
Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro |
| 作者： |
Gui, Lang; Zhang, Peipei; Zhang, Qiya; Zhang, Junbin |
| 出版刊物： |
FISH & SHELLFISH IMMUNOLOGY |
| 出版日期： |
MAR |
| 出版年份： |
2016 |
| 卷/期： |
|
| DOI： |
10.1016/j.fsi.2016.01.038 |
| 论文摘要： |
Hepcidins are small cysteine-rich antimicrobial peptides that play an important role in host immunity against pathogenic organisms. In this study, two hepcidins, SA-hepcidin1 and SA-hepcidin2, were cloned from spotted scat (Scatophagus argus), and the tissue distributions of SA-hepcidins were determined. In addition, mature SA-hepcidin peptides were synthesized to allow evaluation of their antimicrobial and antiviral functions in vitro. SA-hepcidinl belongs to the HAMP1 class and is widely expressed in all tested tissues from spotted scat, whereas SA-hepcidin2 belongs to the HAMP2 class and present only in the liver. The synthetic SA-hepcidins had similar levels of antibacterial activity against Gram-positive and Gram-negative bacteria; however, the antibacterial activity of SA-hepcidinl was stronger than that of SA-hepcidin2. The antiviral activities of the synthetic SA-hepcidins were assessed against Siniperca chuatsi rhabdovirus (SCRV) and largemouth bass Micropterus salmoides reovirus (MsReV) in epithelioma papulosum cyprini (EPC) and grass carp fin (GCF) cells. SA-hepcidin2 had antiviral activity, but SA-hepcidinl did not. The results of this study suggest that SA-hepcidins are important multifunctional proteins in the spotted scat immune system that are involved in resistance to various pathogens. (C) 2016 Elsevier Ltd. All rights reserved. |
