论文
论文标题: Effects of food restriction on growth, body composition and gene expression related in regulation of lipid metabolism and food intake in grass carp
作者: Gong, Yulong; Chen, Weijun; Han, Dong; Zhu, Xiaoming; Yang, Yunxia; Jin, Junyan; Liu, Haokun; Xie, Shouqi
出版刊物: AQUACULTURE
出版日期: FEB 20
出版年份: 2017
卷/期:
DOI: 10.1016/j.aquaculture.2016.12.003
论文摘要: It is well known that most fish would prefer to use body lipid stores for energy expenditure when receiving a long-term food restriction. However, the mechanism of this is still not clear. In the present study, a growth experiment was carried out to investigate the effects of food restriction on growth performance, gene expression related in regulation of lipid metabolism and food ingestion in grass carp (Ctenopharyngodon idellus). Four rations, satiation (S), 80% S, 60% S and 40% S, were adopted in this study. Each treatment was randomly assigned to triplicate net cages of 15 fish (177.3 +/- 3.3 g) per cage. The experiment lasted for 49 days at 30.0 +/- 3.0 degrees C. The experimental results showed that a significant increase in feeding rate and weight gain was found in grass carp with the increased ration level. The body lipid and energy content of the grass carp exhibited a significant decrease when receiving food restriction. The transcriptional levels of the genes involved in lipogenesis (srebp-1c, fas, ppar gamma) were down-regulated at the rations of food restriction. The relative expression of hepatic fas (fatty acid synthetase) and srebp-1c (sterol regulatory element-binding protein 1c) in the fish at satiation were significantly higher than the restricted-fed groups. Similarly, the expressions of hepatic ppar. (peroxisome proliferator-activated receptor-gamma) in the fish at the ration of satiation and 80% S were significantly higher than the group at the low ration of 40% S. However, the expression of hepatic cpt-1a (carnitine palmitoyl transferase I) involved in fatty acid beta-oxidation in fish was significantly up-regulated when receiving food restriction. Other hepatic lipolysis genes of ppar alpha (peroxisome proliferators-activated receptor alpha) and hl (hepatic lipase) didn't show any significant changes in restricted-fed fish. The transcriptional levels of hepatic leptin and hypothalamus pomc (proopiomelanocortin) were significantly down-regulated in fish fed with restricted rations. But the hypothalamus npy (neuropeptide Y) and lepr (leptin receptor) had no change. The present results indicated that a long-term food restriction could cause less accumulation of lipid and could be through a way of down-regulating lipogenesis genes and up-regulating lipolysis genes. Long-term restriction could also activate the appetite of grass carp by down-regulating some anorexigenic genes. Statement of relevance: Food restriction for some time could lead to a suitable lipid storage, in case of accumulation of fatty acid profile and lipid, in cultured grass carp. (C) 2016 Elsevier B.V. All rights reserved.
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  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
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  • “一带一路”海域赤潮数据库
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  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
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