论文
论文标题: Two biomass preparation methods provide insights into studying microbial communities of intestinal mucosa in grass carp (Ctenopharyngodon idellus)
作者: Ngoc Tuan Tran; Xiong, Fan; Hao, Yao-Tong; Zhang, Jing; Wu, Shan-Gong; Wang, Gui-Tang
出版刊物: AQUACULTURE RESEARCH
出版日期: AUG
出版年份: 2017
卷/期: 8
DOI: 10.1111/are.13248
论文摘要: Animal digestive tract is habitat for a large number of autochthonous microbiota, which play central roles in multiple biological and physiological processes of the host. In this study, two different micro-biomass preparation methods were employed to evaluate the diversity of intestinal mucosa-associated microbiota in grass carp (Ctenopharyngodon idellus). Genomic DNAs were isolated either directly from intestinal mucosal samples (group A), or from micro-biomass after microbial dissociation (group B). Community richness, diversity and evenness indices were all higher in group B, but differences were not statistically significant (P=0.97, P=0.33, P=0.34 respectively). Furthermore, group B samples exhibited an increased ratio of bacterial DNA in comparison with group A samples, but the difference was also not statistically significant (P=0.74). In addition, there were no statistically significant differences between the two groups (P>0.05) at the taxonomic level. Our results support previous findings that there exists a great abundance of the intestinal mucosa-adherent microbiota in the grass carp; among these, Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Spirochaetes and Fusobacteria were the most common phyla. Within these microbiota, Paenibacillus, Bacteroides, Bacillus and Cetobacterium genera comprise the majority of the community, implicating their functional importance (e.g. as probiotics) to their host. Our results contribute towards a better understanding of the intestinal microbial profile of grass carp. Both micro-biomass preparation techniques proved to be feasible for studying mucosa-adherent microbiota of grass carp; however, the second method (group B) provides a protocol that is somewhat more effective than the first method (group A).
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 淡水生态与生物技术国家重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
  • 农业农村部
  • 科学技术部
  • 生态环境部
  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅