论文
论文标题: Effects of dietary leucine levels on growth, tissue protein content and relative expression of genes related to protein synthesis in juvenile gibel carp (Carassius auratus gibelio var. CAS III)
作者: Zou, Tao; Cao, Shen-Ping; Xu, Wen-Jie; Han, Dong; Liu, Hao-Kun; Yang, Yun-Xia; Jin, Jun-Yan; Zhu, Xiao-Ming; Xie, Shou-Qi
出版刊物: AQUACULTURE RESEARCH
出版日期: JUN
出版年份: 2018
卷/期: 6
DOI: 10.1111/are.13682
论文摘要: An 8-week feeding trial was conducted to investigate the impacts of dietary leucine on growth, hepatopancreas and muscle protein content and relative expressions of protein synthesis-related genes in juvenile gibel carp (5.2 g). Triplicate tanks of fish (22 fish per tank) were fed with a low (0.82%), middle (1.90%) or high (2.99%) leucine diet to apparent satiation three times a day. The results showed that the weight gain (WG) and feed efficiency (FE) of the fish fed with the 1.90% leucine diet had the highest values compared to those of other two groups (p < .05). Dietary leucine significantly affected the protein and lipid contents of the hepatopancreas (p < .05), but not muscle or the whole body composition of the fish. The amino acid contents (Arginine, Leucine, Methionine, Phenylalanine, Alanine, Aspartic acid, Glutamic acid and Proline) of the dorsal muscles of fish fed with a 1.90% leucine diet were significantly higher than those fed with a 0.82% leucine diet. Moreover, transcriptional levels of target genes of rapamycin (TOR), ribosomal protein S6 kinase 1 (S6K1), eukaryotic translation initiation factor 4E (eIF4E) and leucyl-tRNA synthetase (LARS) in the hepatopancreas were significantly up-regulated (p < .05), but eukaryotic translation initiation factor 4E-binding protein 2 (4E-BP2) was significantly down-regulated (p < .05) with the increasing dietary leucine levels. However, dietary leucine had no effects on the gene expressions of 4E-BP2 and S6K1 in the muscle of gibel carp. The present results indicate that dietary leucine led to a marked tissue-specific response on protein content in the muscle and hepatopancreas of gibel carp that is related to the TOR signalling pathway.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
  • 农业农村部
  • 科学技术部
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  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅