论文
论文标题: High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii
作者: Miao, Rongli; Ma, Xiaocui; Deng, Xuan; Huang, Kaiyao
出版刊物: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
出版日期: MAR
出版年份: 2019
卷/期:
DOI: 10.1016/j.algal.2018.101400
论文摘要: Many microalgae accumulate large amounts of lipid droplets (LDs) following N deprivation. The triacylglycerols (TAGs) in these LDs can be used as a feedstock to produce biodiesel. To investigate the molecular mechanism underlying LD formation, we used a percoll gradient-based method to enrich for mutants with defects in LD formation and generated an insertional mutagenesis library containing > 11,000 Chlamydomonas reinhardtii transformants. One of the mutants harbored a mutation in a gene encoding a glutathione peroxidase (GPX5) that catalyzes the formation of water from hydrogen peroxide (H2O2) or organic hydroperoxide. The gpx5 mutant had a 35% reduction in the number of LDs and a 50% reduction in TAG content compared with the parental strain CC4348 at 24 h of N deprivation. The full-length of GPX5 or truncate GPX5 without the putative signal peptide or the putative signal peptide of GPX5 fused YFP localized in the cytoplasm, but not in chloroplasts. Complementation with full-length GPX5 rescued the mutant phenotypes, and the expression of GPX5 in a complemented strain L27 was increased 2-3 fold after 24 h of N deprivation. Moreover, the gpx5 mutant showed increased sensitivity to the singlet oxygen (O-1(2)) stress generated by the photosensitizer Rose Bengal (RB). The ROS concentration of the gpx5 was about 1.5 times that of CC4348 during N deprivation. Artificial increasing of ROS concentration in cells abolished the formation of LDs in Chlamydomonas. These data suggest that a high level of ROS attenuates LD formation.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
  • 农业农村部
  • 科学技术部
  • 生态环境部
  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅