| 论文标题： |
Cloning and characterization of the LEF/TCF gene family in grass carp (Ctenopharyngodon idella) and their expression profiles in response to grass carp reovirus infection |
| 作者： |
Zhu, Denghui; Huang, Rong; Chen, Liangming; Fu, Peipei; Luo, Lifei; He, Libo; Li, Yongming; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping |
| 出版刊物： |
FISH & SHELLFISH IMMUNOLOGY |
| 出版日期： |
MAR |
| 出版年份： |
2019 |
| 卷/期： |
|
| DOI： |
10.1016/j.fsi.2018.11.057 |
| 论文摘要： |
T-cell factor/lymphoid enhancer-binding factor (TCF/LEF) proteins from the High Mobility Group (HMG) box family act as the main downstream effectors of the Wnt signaling pathway. HMGB proteins play multifaceted roles in the immune system of mammals. To clarify the immunological characteristics of LEF/TCF genes in grass carp (Ctenopharyngodon idella), five LEF/TCF genes (TCF7, LEF1, TCF7L1A, TCF7L1B, and TCF7L2) were identified and characterized. All five LEF/TCF proteins contained two characteristic domains: a HMG-BOX domain and a CTNNB1_binding region. Phylogenetic tree analysis revealed that the LEF/TCF proteins were represented different lineages. These results of subcellular localization showed that four of the LEF/TCF genes were localized exclusively within the nucleus, while TCF7L2 was localized in the cytoplasm and nucleus. The mRNA expression profiles of these LEF/TCF family genes differed across different tissues. The mRNA expression levels of TCF7, TCF7L1A, and TCF7L2 changed significantly in liver after grass carp reovirus (GCRV) challenge; TCF7 and TCF7L1A responded early while TCF7L2 responded late. This suggests that these genes may participate in GCRV-related immune responses. Moreover, TCF7 promoted Bc16 transcription in response to the GCRV challenge. These findings further our understanding of the function of LEF/TCF genes in teleosts. |
