论文
论文标题: High-Throughput Sequencing Analysis Reveals Correlations between Host Phylogeny, Gut Microbiota, and Habitat of Wild Frogs from a Mountainous Area
作者: Shu, Yilin; Wang, Gang; Hong, Pei; Yu, Qiang; Zhang, Jihui; Donde, Oscar Omondi; Xiao, Bangding; Wu, Hailong
出版刊物: COPEIA
出版日期: MAR
出版年份: 2019
卷/期: 1
DOI: 10.1643/OT-18-040
论文摘要: Gut microbiota of vertebrate hosts play crucial roles for the host they inhabit. In this study, we compared the composition and predicted functions of gut microbiota of female individuals belonging to four frog species from different habitats using bacterial 16S rRNA gene sequencing. Our results showed that the gut microbiota of the examined frog species were dominated by the phyla Firmicutes, Proteobacteria, Bacteriodetes, Verrucomicrobia, and Fusobacteria. There were significant differences in the relative abundance of dominant phyla among the four species, although the relative abundance of dominant phyla in the microbiota of individuals of Odorrana tormota was more evenly balanced (ranged from 10.45% for Fusobacteria to 27.21% for Bacteroidetes). The alpha diversity patterns varied depending on the microbial taxon level. At the phylum level, the Chao1 diversity was positively associated with body weight, with the diversity index scores of O. tormota and Amolops wuyiensis markedly lower than those of Odorrana schmackeri and Polypedates megacephalus. At the genus level, Chao1 diversity was associated with evolutionary relationships among species, with the Chao1 diversity index score for P. megacephalus of distant evolutionary relationships being significantly higher than the scores for frogs with close evolutionary relationships. Moreover, permutational multivariate analysis of variance revealed significant differences in the gut bacterial community structure among species (R-2 = 0.510, P = 0.001). Further, the predicted functions of the gut microbiota were similar for species with close evolutionary relationships but differed with distant evolutionary relationships. Taken together, our results indicate that wild frogs have species-specific microbial communities and provide insight into the ecological and evolutionary dynamics of host-microbe interactions.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
  • 农业农村部
  • 科学技术部
  • 生态环境部
  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅