| 论文标题： | Investigating the Role of BATF3 in Grass Carp (Ctenopharyngodon idella) Immune Modulation: A Fundamental Functional Analysis |
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| 作者： | Zhu, Denghui; Huang, Rong; Fu, Peipei; Chen, Liangming; Luo, Lifei; Chu, Pengfei; He, Libo; Li, Yongming; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping |
| 出版刊物： | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
| 出版日期： | APR 4 |
| 出版年份： | 2019 |
| 卷/期： | 7 |
| DOI： | 10.3390/ijms20071687 |
| 论文摘要： | Basic leucine zipper transcription factor ATF-like (BATF)-3, belonging to activator protein 1 (AP-1) superfamily transcription factors, is essential for homeostatic development of CD8 alpha(+) classical dendritic cells activating CD8 T-cell responses to intracellular pathogens. In this study, the characteristics and cDNA cloning of the CiBATF3 molecule were described in grass carp (Ctenopharyngodon idella). CiBATF3 had abundant expression in immune-related organizations, including liver, spleen and gill, and grass carp reovirus (GCRV) infection had significantly changed its expression level. After Ctenopharyngodon idella kidney (CIK) cells were challenged with pathogen-associated molecular patterns (PAMPs), polyinosinic:polycytidylic acid (poly(I:C)) stimulation induced higher mRNA levels of CiBATF3 than that of lipopolysaccharide (LPS). Subcellular localization showed that CiBATF3-GFP was entirely distributed throughout cells and nuclear translocation of CiBATF3 was found after poly(I:C) treatment. Additionally, the interaction between CiBATF3 and interleukin 10 (IL-10) was proven by bimolecular fluorescence complementation (BiFC) system. The small interfering RNA (siRNA)-mediated CiBATF3 silencing showed that the mRNA of CiBATF3 and its downstream genes were down-regulated in vitro and in vivo. CiBATF3 played a negative regulatory role in the transcriptional activities of AP-1 and NF-kappa B reporter gene. In summary, the results may provide valuable information on fundamental functional mechanisms of CiBATF3. |
