论文
论文标题: PfHMGB2 protects yellow catfish (Pelteobagrus fulvidraco) from bacterial infection by promoting phagocytosis and proliferation of PBL
作者: Wang, Yun; Yang, Yanyan; Chen, Qianying; Zhai, Hanfei; Xie, Zhaohui; Ke, Fei
出版刊物: FISH & SHELLFISH IMMUNOLOGY
出版日期: OCT
出版年份: 2019
卷/期:
DOI: 10.1016/j.fsi.2019.08.007
论文摘要: HMGB2, a member of the high mobility group box family, plays an important role in host immune responses. However, the mechanism of action of HMGB2 is not well understood. Herein, a homologue from yellow catfish (Pelteobagrus fulvidraco) was cloned and named PfHMGB2. The deduced amino acid sequence of PfHMGB2 possessed a typical tripartite structure (two DNA binding boxes and an acid tail) and shared 90% identity with the predicted HMGB2 from I. punctatus. The mRNA of PfHMGB2 was widely distributed in all 11 tested tissues in healthy fish bodies and was significantly induced in the liver and head kidney when yellow catfish were injected with inactivated Aeromonas hydrophila. Consistently, PfHMGB2 mRNA could also be induced in yellow catfish peripheral blood leucocytes (PBL) by lipopolysaccharide. The recombinant PfHMGB2 protein was purified from E. coli BL21 (DE3):pET-28a/PfHMGB2 and showed DNA-binding affinity. Moreover, rPfHMGB2 improved the phagocytosis and proliferation activity and upregulated the mRNA expression of the pro-inflammatory cytokine TNFa in yellow catfish PBL. These results indicated that PfHMGB2 could protect yellow catfish from pathogen infection by activating PBL.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
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  • == 相关网站推荐 ==
  • 中国科学院
  • 农业农村部
  • 科学技术部
  • 生态环境部
  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅