论文
论文标题: Efficacy of zero nitrous oxide emitting aerobic denitrifying bacterium, Methylobacterium gregans DC-1 in nitrate removal with strong auto-aggregation property
作者: Hong, Pei; Shu, Yilin; Wu, Xingqiang; Wang, Chunbo; Tian, Cuicui; Wu, Hailong; Donde, Oscar Omondi; Xiao, Bangding
出版刊物: BIORESOURCE TECHNOLOGY
出版日期: DEC
出版年份: 2019
卷/期:
DOI: 10.1016/j.biortech.2019.122083
论文摘要: A novel aerobic denitrifying strain Methylobacterium gregans DC-1 was isolated and identified. Strain DC-1 removed 98.4% of nitrate-nitrogen (NO3--N) and 80.7% of total organic carbon with initial concentrations of 50 and 2400 mg/1, respectively. The N balance showed that most NO3--N was converted to N-2(62.18%) without nitrous oxide (N2O) emission. Response surface analysis showed that the optimal conditions for total N removal were carbon (C):N ratio of 18.7, temperature of 26.8 degrees C, pH of 6.5 and shaking speed of 180 rpm. In combination with the N balance and successful amplification of napA, nirK and nosZ genes, the metabolic pathway was as follows: NO3-NO2- -> NO -> N-2(O) -> N-2. Strain DC-1 had strong auto-aggregation rate (maximum 38.7%), produced large amounts of extracellular polymeric substances (EPS; maximum of 781.4 mg/g cell dry weight) and had corresponding strong hydrophobicity (maximum 83.2%). Pearson correlation analysis showed that EPS content and hydrophobicity were significantly positively correlated with auto-aggregation.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
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  • 武汉白暨豚保护基金会
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  • 中国动物学会原生动物学分会
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  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
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  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
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  • == 相关网站推荐 ==
  • 中国科学院
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  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅