| 论文摘要： |
Silver nanoparticles (AgNPs) have been increasingly manufactured and thus are increasingly detected in aquatic systems. However, there are still some overlooked factors (e.g., organism sex) in the field of nanotoxicological assessment. In this study, to explore the role of sex in nanotoxicity, adult male and female zebrafish were exposed to 100 mu g/L of two uncoated commercial AgNPs with primary sizes 20 nm and 80 nm for 2 weeks, after which the impacts of AgNPs on intestines and livers of both male and female zebrafish were assessed using a suite of biomarkers. Results demonstrated that the intestinal Na/KATPase activity as well as the superoxide dismutase activity in male zebrafish differed significantly between 20-nm AgNPs and 80-nm AgNPs treatments (p < 0.05), indicating 20-nm AgNPs showing higher toxicity to zebrafish than the 80-nm AgNPs. Also, we noted that the used AgNPs induced sex-dependent effects on growth indices, oxidative/anti-oxidative status, neural signaling and hepatic lipid metabolism, with the male zebrafish being more sensitive to AgNPs than the females. Further, the tested AgNPs impaired the intestine much more seriously than the liver, as evidenced by the disruptions of Na/KATPase and antioxidant system in intestine but not in liver. These findings imply that prolonged exposure to AgNPs might induce size-related, sex-dependent, and organ-specific toxicity to adult zebrafish, thereby may significantly extend our understanding of the toxic effects of AgNPs in aquatic environment. (C) 2020 Elsevier Ltd. All rights reserved. |
