| 论文摘要： |
Geosmin (trans-1, 10-dimethyl-trans-9-decalol), a volatile organic compound, has been widely detected in aquatic ecosystems. However, the ecological effects of geosmin are not clear. Here, using zebrafish (Danio rerio) embryo as a model, we investigated biological activity effects of environmentally relevant concentrations (50, 500, 5000 ng/L) of geosmin on the developing zebrafish starting from 2 h post-fertilization (hpf) to 96 hpf. Results showed geosmin had no effect on hatchability, malformations and mortality. However, we observed that geosmin exposure significantly increased zebrafish body length in a concentration dependent manner. This effect was possibly due to up-regulation of expression of genes along the growth hormone/insulin-like growth factor (GH/IGF) axis and hypothalamic-pituitary-thyroid (HPT) axis. In addition, superoxide dismutase (SOD) activities and catalase (CAT) activities significantly increased at 96 hpf when the embryos were exposed to 500 and 5000 ng/L of geosmin. The malondialdehyde (MDA) contents and glutathione S-transferase (GST) activities decreased significantly after the exposure to 5000 ng/L geosmin. Simultaneously, exposure to geosmin resulted in significant increase in cell apoptosis, mainly in the heart area. The mRNA levels of the genes related to oxidative stress and apoptosis were also altered significantly after geosmin exposure. These findings indicated that geosmin can simultaneously induce multiple responses during zebrafish embryonic development, including oxidative stress, apoptosis, and endocrine disruption. |
