| 论文标题： |
Temperature effects on SVCV propagation and the related IFN response in zebrafish |
| 作者： |
Zhou, Xiao-yu; Lu, Long-feng; Li, Zhuo-cong; Zhang, Can; Chen, Dan-dan; Li, Shun |
| 出版刊物： |
AQUACULTURE |
| 出版日期： |
FEB 25 |
| 出版年份： |
2021 |
| 卷/期： |
|
| DOI： |
10.1016/j.aquaculture.2020.736084 |
| 论文摘要： |
Significant cyprinid fish mortality often occurs around late spring and early summer. The reason for this phenomenon remains unclear, especially the molecular mechanism. Whether the fish immune response is weaker or the viral pathogenicity is higher during these seasons needs to be clarified. Experiments were conducted at 16, 22 and 28 degrees C to imitate the environmental conditions experienced by fish and investigate the influence of temperature on the process of fish virus infection. The host antiviral response and the virus replication ability were strongest at 22 degrees C and the results indicated that the virus breaks through the fish antiviral defense through efficient proliferation. Both cultured cells and fish were substantially more susceptible to spring viremia of carp virus (SVCV) infection at 22 degrees C than at 16 degrees C or 28 degrees C. The highest transcription and translation levels for both the host interferon (IFN)-related and viral genes were observed at 22 degrees C. Further, the interactions of SVCV N and P proteins were also highest at 22 degrees C. Collectively, our data suggest that 22 degrees C is the optimal temperature for SVCV replication. Despite the extremely robust host IFN response at this temperature, the virus exhibited the highest pathogenicity. |
