论文
论文标题: Development and application of quantitative real-time PCR based on the mitochondrial cytochrome oxidase subunit I gene for early detection of the grazer Poterioochromonas malhamensis contaminating Chlorella culture
作者: Wang, Xianhui; Li, Huannan; Zhan, Xueling; Ma, Mingyang; Yuan, Danni; Hu, Qiang; Gong, Yingchun
出版刊物: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
出版日期: MAR
出版年份: 2021
卷/期:
DOI: 10.1016/j.algal.2020.102133
论文摘要: Poterioochromonas malhamensis is a flagellate grazer that readily contaminates Chlorella cultures and can have a devastating impact on them, especially in large-scale culture. Here, we developed a quantitative real-time polymerase chain reaction (qPCR) method to detect and quantify P. malhamensis from high-density Chlorella cultures. A set of primers specific to the mitochondrial cytochrome oxidase subunit I gene (COI) of P. malhamensis was screened and characterized comprehensively for specificity, sensitivity and applicability to the field samples. The results showed that the primers were sufficiently specific to distinguish P. malhamensis from a wide variety of micro-eukaryotes which often coexist with P. malhamensis in microalgal cultures, and could detect a very low concentration of P. malhamensis from high concentrations of Chlorella sorokiniana (10(6), 10(7), and 10(8) C. sorokiniana cells mL(-1)). Moreover, the qPCR method was applied successfully to the monitoring of P. malhamensis population dynamics in Chlorella cultures in 100 L open ponds. Finally, the qPCR method was applied to the detection of this flagellate in the wider environment of the culture facility. It showed that the flagellate was widely distributed in the environment, and we speculate that Poterioochromonas in Chlorella cultures could originate from the air, the water, and the seed cultures. The qPCR method developed therefore proved to have the specificity and efficiency to be useful for early detection of P. malhamensis in industrial-scale Chlorella cultivation.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
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  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
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