| 论文标题： |
Identification and functional characterization of a short-type peptidoglycan recognition protein, PGRP-S in the orange-spotted grouper, Epinephelus coioides |
| 作者： |
Hou, Jing; Gan, Zhen; Chen, Shannan; Cheng, Jun; Lu, Yishan; Huang, Bei; Nie, Pin; Xia, Liqun |
| 出版刊物： |
AQUACULTURE REPORTS |
| 出版日期： |
JUL |
| 出版年份： |
2021 |
| 卷/期： |
|
| DOI： |
10.1016/j.aqrep.2021.100739 |
| 论文摘要： |
Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) mediating the recognition of peptidoglycan. In the present study, a short-type PGRP gene named Eco-PGRP-S was identified in the orange-spotted grouper (Epinephelus coioides), a major commercial marine fish cultured in Asia. Eco-PGRP-S gene was constitutively expressed in all tissues examined, with the highest expression level observed in gill and skin. As a typical PRR, Eco-PGRP-S is inducible following the stimulation of peptidoglycan, and has an ability to bind peptidoglycan. Importantly, Eco-PGRP-S has been proven to have Zn2+-dependent amidase activity and direct antibacterial activity against Vibrio Harveri and Edwardsiella tarda. In addition, Eco-PGRP-S is mainly localized in cytoplasm and nucleus, and is able to significantly trigger the activation of NF-kappa B signalling. The present research thus contributes to a better understanding of the molecular mechanism of antibacterial defense and the containment of infectious bacterial diseases in grouper. |
