| 论文摘要： |
Perfluorobutanesulfonate (PFBS) is an emerging pollutant of international concern, which is found to impair the early embryonic development of fishes. In the context of ubiquitous and persistent pollution, it is necessary to explore mitigatory strategies against the developmental toxicity of PFBS. In this study, zebrafish larvae were acutely exposed to 0, 1, 3.3 and 10 mg/L of PFBS till 168 h post-fertilization (hpf), during which probiotic Lactobacillus rhamnosus bacteria were administered via the exposure media. After the singular or combined exposure, interaction between PFBS and probiotics on the growth of zebrafish larvae was measured. PFBS exposure significantly decreased the larval body weight, weight gain and specific growth rate, while probiotic supplementation efficiently inhibited the growth retardation caused by PFBS. Furthermore, PFBS and probiotic combinations remarkably activated the antioxidant capacity to timely scavenge the reactive oxidative species and protect the larvae from lipid peroxidation. Biochemical assay and fluorescent staining verified that PFBS exposure significantly promoted the production of bile acids, which were further enhanced by the probiotics. In coexposed zebrafish larvae, up-regulation of peroxisome proliferator-activated receptor (PPARb) would enhance the beta-oxidation of fatty acids to meet the energy demand from larval growth, subsequently decreasing fatty acid concentrations. In addition, probiotic supplements masked the dysbiosis of PFBS and potently shaped the gut microbiota, which closely modulated the production of bile acids. Overall, the present findings underline the beneficial effects of probiotics to protect the developing larvae from the aquatic toxicities of PFBS, thus highlighting the potential application values of probiotic recipe in aquaculture and ecological reservation. (c) 2021 Elsevier B.V. All rights reserved. |
