| 论文标题： |
Heterozygous depletion of pik3r1 improves growth and feed conversion efficiency in Gibel carp (Carassius gibelio) |
| 作者： |
Huang, Jianfei; Shi, Chuang; Gao, Yanping; Su, Jingzhi; Shu, Yuqin; Zeng, Ningmei; Zhai, Gang; Jin, Xia; Han, Dong; He, Jiangyan; Lou, Qiyong; Yin, Zhan |
| 出版刊物： |
AQUACULTURE |
| 出版日期： |
DEC 15 |
| 出版年份： |
2021 |
| 卷/期： |
|
| DOI： |
10.1016/j.aquaculture.2021.737207 |
| 论文摘要： |
Phosphatidylinositol 3-kinase (PI3K) signaling plays an indispensable role in vertebrate metabolism and energy homeostasis. Heterozygous disruption of P85 alpha, a PI3K subunit encoded by the pik3r1 gene, is known to improve insulin sensitivity in mammals. However, there is no direct evidence on the effects of altered expression of pik3r1 on metabolism and somatic growth in teleosts. In this study, the effects of heterozygous disruption of pik3r1 on metabolism and somatic growth in Gibel carp were investigated. Enhancement of the PI3K/AKT/mTOR signaling pathway was achieved by inducing haploinsufficiency of P85 alpha in heterozygous pik3r1-deficient Gibel carp. Unlike in mammalian models, there was no significant changes in the plasma glucose levels and hepatic glucose uptake. However, significant reduction in the levels of plasma triglycerides (TG), hepatic TG, amino acids, and lactate was observed. Furthermore, the haploinsufficiency of P85 alpha significantly improved somatic growth, lipid utilization, and feed conversion efficiency (FCE) in the mutant Gibel carp. Taken together, our results demonstrate that enhancement of the PI3K/AKT/mTOR signaling pathway could effectively improve somatic growth and FCE in practical aquaculture. Therefore, genetic engineering for partial activation of the PI3K signaling pathway may be a potential breeding strategy for improvement of the traits of cultured fish in the future. |
