论文
论文标题: Heterozygous depletion of pik3r1 improves growth and feed conversion efficiency in Gibel carp (Carassius gibelio)
作者: Huang, Jianfei; Shi, Chuang; Gao, Yanping; Su, Jingzhi; Shu, Yuqin; Zeng, Ningmei; Zhai, Gang; Jin, Xia; Han, Dong; He, Jiangyan; Lou, Qiyong; Yin, Zhan
出版刊物: AQUACULTURE
出版日期: DEC 15
出版年份: 2021
卷/期:
DOI: 10.1016/j.aquaculture.2021.737207
论文摘要: Phosphatidylinositol 3-kinase (PI3K) signaling plays an indispensable role in vertebrate metabolism and energy homeostasis. Heterozygous disruption of P85 alpha, a PI3K subunit encoded by the pik3r1 gene, is known to improve insulin sensitivity in mammals. However, there is no direct evidence on the effects of altered expression of pik3r1 on metabolism and somatic growth in teleosts. In this study, the effects of heterozygous disruption of pik3r1 on metabolism and somatic growth in Gibel carp were investigated. Enhancement of the PI3K/AKT/mTOR signaling pathway was achieved by inducing haploinsufficiency of P85 alpha in heterozygous pik3r1-deficient Gibel carp. Unlike in mammalian models, there was no significant changes in the plasma glucose levels and hepatic glucose uptake. However, significant reduction in the levels of plasma triglycerides (TG), hepatic TG, amino acids, and lactate was observed. Furthermore, the haploinsufficiency of P85 alpha significantly improved somatic growth, lipid utilization, and feed conversion efficiency (FCE) in the mutant Gibel carp. Taken together, our results demonstrate that enhancement of the PI3K/AKT/mTOR signaling pathway could effectively improve somatic growth and FCE in practical aquaculture. Therefore, genetic engineering for partial activation of the PI3K signaling pathway may be a potential breeding strategy for improvement of the traits of cultured fish in the future.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
  • 农业农村部
  • 科学技术部
  • 生态环境部
  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅