论文
论文标题: Photosynthetic Conversion of CO2 Into Pinene Using Engineered Synechococcus sp. PCC 7002
作者: Yang, Ruigang; Zhu, Lingyun; Li, Tao; Zhu, Lv-yun; Ye, Zi; Zhang, Dongyi
出版刊物: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
出版日期: DEC 17
出版年份: 2021
卷/期:
DOI: 10.3389/fbioe.2021.779437
论文摘要: Metabolic engineering of cyanobacteria has received much attention as a sustainable strategy to convert CO2 to various longer carbon chain fuels. Pinene has become increasingly attractive since pinene dimers contain high volumetric energy and have been proposed to act as potential aircraft fuels. However, cyanobacteria cannot directly convert geranyl pyrophosphate into pinene due to the lack of endogenous pinene synthase. Herein, we integrated the gene encoding Abies grandis pinene synthase into the model cyanobacterium Synechococcus sp. PCC 7002 through homologous recombination. The genetically modified cyanobacteria achieved a pinene titer of 1.525 +/- 0.l45 mg L-1 in the lab-scale tube photobioreactor with CO2 aeration. Specifically, the results showed a mixture of alpha- and beta-pinene (similar to 33:67 ratio). The ratio of beta-pinene in the product was significantly increased compared with that previously reported in the engineered Escherichia coli. Furthermore, we investigated the photoautotrophic growth performances of Synechococcus overlaid with different concentrations of dodecane. The work demonstrates that the engineered Synechococcus is a suitable potential platform for beta-pinene production.
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  • 中国科学院藻类生物学重点实验室
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  • 水生生物博物馆
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