| 作者： |
Xu, Wenjie; Geng, Hancheng; Wu, Liyun; Jin, Junyan; Han, Dong; Zhu, Xiaoming; Yang, Yunxia; Liu, Haokun; Xie, Shouqi |
| 论文摘要： |
To investigate the physiological responses to different energy sources under feeding restriction in fish, gibel carp were fed a high lipid, low carbohydrate diet (HL diet, 12% lipid, 16% carbohydrate) or a high carbohydrate, low lipid diet (HC diet, 34% carbohydrate, 4% lipid) to satiation or 70% satiation (restriction) for 56 d; then, growth performance, nutrient metabolism, endoplasmic reticulum (ER) stress, autophagy, and apoptosis were determined. Fish that were fed the HL diet had higher final body weight, specific growth rate, and feed efficiency, lower phosphorylation levels of AKT and mTOR proteins and lower mRNA levels of gk than the HC group. When fed to 70% satiation, fish had lower feed intake, feeding rate, final body weight, and specific growth rate, and lower plasma glucose and nonesterified fatty acid levels. Feeding restriction induced the transcript level of gk and suppressed the mRNA level of acly, acc, fas. There were no significant differences in ER stress, autophagy and apoptosis between feeding levels or diets. Interactions between feeding level and diet were observed in plasma triglyceride level and the gene expression levels of fbpase and srebp1. Thus, gibel carp utilized HL diet better than HC diet, which was unalerted by feeding restriction. |
