论文
论文标题: The combined effect of acute hypoxic stress and feeding status on the metabolism of yellow catfish (Pelteobagrus fulvidraco)
作者: Wang, Jie; Wang, Man; Li, Bo; Guo, Hongxi; Zhu, Xiaoming; Zhang, Lei
出版刊物: AQUACULTURE
出版日期: NOV 15
出版年份: 2022
卷/期:
DOI: 10.1016/j.aquaculture.2022.738605
论文摘要: Metabolism is one of the most important physiological processes in fish that changes daily depending on the variability of disparate factors. Dissolved oxygen and feeding status are critical to the growth and metabolism of fish. In this study, the effects of dissolved oxygen and feeding status, as well as their interaction on the blood biochemistry and metabolism of yellow catfish (Pelteobagrus fulvidraco) were investigated using a two-factor test. At a dissolved oxygen content of 1.2 mg mL(-1), yellow catfish were exposed to hypoxia for 48 h and divided into four experimental groups: normoxia (6.5 mg L-1)-fasting group (C; Control group); normoxia-feeding group (F); hypoxia (1.2 mg L-1)-fasting group (H); and hypoxia-feeding group (FH). Analysis revealed that both hypoxia and feeding caused a short-term increase in the number of red blood cells and hemoglobin concentration in yellow catfish, thereby increasing the oxygen-carrying capacity of the blood. After feeding, the metabolic activity in yellow catfish showed a trend of rising and then falling. Following hypoxia, yellow catfish activated anaerobic glycolysis to provide the energy required by the body, and the mode of metabolism in yellow catfish gradually converted from aerobic to anaerobic. Moreover, the activity of respiratory metabolism enzyme succinate dehydrogenase (SDH) tended to decrease and then increase, while that of lactate dehydrogenase (LDH) tended to increase and then decrease, showing opposing trends. Furthermore, the liver glycogen was mobilized, blood glucose levels increased, and lactic acid accumulation occurred in the short term. Meanwhile, the hypoxia-inducible factor (hif-1 alpha) was activated, the expression of glucose metabolism-related genes (epo, hk1, pfkla, pkma, and gk) dramatically increased, and the activities of the three key enzymes involved in glycolysis (HK, PFK, and PK) were elevated for a short period of time. In conclusion, both hypoxia and feeding altered blood biochemical and metabolic responses in yellow catfish over time, and the metabolic responses were more intense in the post-feeding hypoxia group.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
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  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
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  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅