论文标题: |
Critical assessment of protozoa contamination and control measures in mass culture of the diatom Phaeodactylum tricornutum |
作者: |
He, Qing; Zhang, Hanwen; Ma, Mingyang; He, Yue; Jia, Jing; Hu, Qiang; Gong, Yingchun |
出版刊物: |
BIORESOURCE TECHNOLOGY |
出版日期: |
SEP |
出版年份: |
2022 |
卷/期: |
|
DOI: |
10.1016/j.biortech.2022.127460 |
论文摘要: |
The diatom Phaeodactylum tricornutum is considered a promising cell factory for high-value products fucoxanthin and EPA. But its potential has not been realized due largely to microbial contamination. In this study, seven protozoan strains were identified, of which a heterolobosean amoeba was identified as the most frequently occurring and destructive predator in P. tricornutum culture. The addition of 400 mg L-1 NH4HCO3 inhibited amoeba proliferation with little impact on algal growth. Halting culture mixing at night induced a hypoxia environment that further inhibited amoeba growth. Regardless of culture systems employed, a periodical supply of proper amounts of NH4HCO3 alone or in combination with halting culture mixing at night may prevent or treat protozoa contamination in mass culture of P. tricornutum. |