| 论文标题： |
Cilia regulate meiotic recombination in zebrafish |
| 作者： |
Xie, Haibo; Wang, Xiaosi; Jin, Minjun; Li, Lanqin; Zhu, Junwen; Kang, Yunsi; Chen, Zhe; Sun, Yonghua; Zhao, Chengtian |
| 出版刊物： |
JOURNAL OF MOLECULAR CELL BIOLOGY |
| 出版日期： |
SEP 27 |
| 出版年份： |
2022 |
| 卷/期： |
|
| DOI： |
10.1093/jmcb/mjac049 |
| 论文摘要： |
Meiosis is essential for evolution and genetic diversity in almost all sexual eukaryotic organisms. The mechanisms of meiotic recombination, such as synapsis, have been extensively investigated. However, it is still unclear whether signals from the cytoplasm or even from outside of the cell can regulate the meiosis process. Cilia are microtubule-based structures that protrude from the cell surface and function as signaling hubs to sense extracellular signals. Here, we reported an unexpected and critical role of cilia during meiotic recombination. During gametogenesis of zebrafish, cilia were specifically present in the prophase stages of both primary spermatocytes and primary oocytes. By developing a germ cell-specific CRISPR/Cas9 system, we demonstrated that germ cell-specific depletion of ciliary genes resulted in compromised double-strand break repair, reduced crossover formation, and increased germ cell apoptosis. Our study reveals a previously undiscovered role for cilia during meiosis and suggests that extracellular signals may regulate meiotic recombination via this particular organelle. |
