| 论文摘要： |
Ammonia is recognized as an environmental stressor for fish. As resveratrol (RES) has anti-inflammatory and antioxidant properties, we hypothesized that RES could attenuate the response to ammonia exposure in gibel carp. Therefore, gibel carp were fed a diet containing RES for eight weeks, followed by acute ammonia stimu-lation. Stress induced by acute ammonia exposure could be ameliorated by RES, manifested by down-regulated plasma glucose, and up-regulated C3 and IgM levels. Furthermore, decreased AST and LDH; enhanced T-AOC, SOD, and GPx in the liver; and reduced damage to gill and liver tissues indicated that RES attenuated oxidative and tissue damage induced by ammonia exposure. Moreover, RES activated the Nrf2/HO-1 pathway and up -regulated the expression of several antioxidant genes. RES enhanced anti-inflammatory activity as reflected by activation of the NF-kappa B pathway, down-regulated the expression of pro-inflammatory cytokines (nf kappa b, tnf-alpha, and il-1 beta), and up-regulated the expression of anti-inflammatory cytokines (il-4 and il-10). In terms of mitochondrial function, RES up-regulated protein levels of p-AMPK, SIRT1, and PGC-1 alpha; inhibited mitochondrial fission; pro-moted mitochondrial fusion and biogenesis-related gene expression. Overall, the results suggest that RES mediated the Nrf2/HO-1, NF-kappa B, and AMPK/SIRT1/PGC-1 alpha pathways to attenuate oxidative stress, inflamma-tion, and mitochondrial dysfunction induced by ammonia in gibel carp. |
