| 论文标题： |
Geosmin disrupts energy metabolism and locomotor behavior of zebrafish in early life stages |
| 作者： |
Zhou, Weicheng; Wang, Yuming; Wang, Jinglong; Peng, Chengrong; Wang, Zhicong; Qin, Hongjie; Li, Genbao; Li, Dunhai |
| 出版刊物： |
SCIENCE OF THE TOTAL ENVIRONMENT |
| 出版日期： |
FEB 10 |
| 出版年份： |
2023 |
| 卷/期： |
|
| DOI： |
10.1016/j.scitotenv.2022.160222 |
| 论文摘要： |
Geosmin has been commonly detected both in various aquatic environments and biota, but its exact toxicological mechanisms to organisms need further experimentation. In the present study, zebrafish embryos were exposed to geosmin at nominal concentrations of 50, 500 and 5000 ng/L for 120 h post-fertilization (hpf), followed by locomotor activity and biochemical parameter examination, and multi-omics investigation of the transcriptome and metabolome. The results showed that geosmin exposure significantly reduced the mitochondrial electron transport chain (ETC) complexes I-V, ATP content and mitochondrial respiration and suppressed the locomotor behavior of zebrafish larvae. Transcriptomics analysis revealed that the transcripts of genes involved in oxidative phosphorylation, glycolysis, and lipid metabolism were significantly affected, indicating that geosmin disrupts energy metabolism. Furthermore, meta-bolomics results showed that 3 classes of lipids, namely glycerophospholipids (GPs), sphingolipids (SLs) and fatty acyls (FAs) were significantly decreased after geosmin exposure. This study provides novel insight into the underlying mech-anisms of geosmin-induced energy metabolism and highlights the need for concern about geosmin exposure. |
