论文
论文标题: Nitrogen and phosphorus turnover and coupling in ponds with different aquaculture species
作者: Bai, Dong; Li, Xiaowen; Liu, Zhenghan; Wan, Lingling; Song, Chunlei; Zhou, Yiyong; Cao, Xiuyun
出版刊物: AQUACULTURE
出版日期: JAN 30
出版年份: 2023
卷/期:
DOI: 10.1016/j.aquaculture.2022.738997
论文摘要: Massive accumulation of nitrogen (N) and phosphorus (P) is the serious problem for development of pond aquaculture. Several efforts have been paid to reduce negative effects of nutrient accumulation. However, little is known about the internal cycling process of N and P in traditional aquaculture ponds. In this study, we determined the kinetic parameters of critical extracellular enzymes (including alkaline phosphatase (AP) and leucine aminopeptidase (LAP)) involved in N and P cycling and variations of trophic states in three kinds of ponds with typical cultured species (fish, crab, and crayfish) around Lake Honghu in four months of different seasons from 2019 to 2020. Additionally, sediment P release potential was assessed meanwhile. Results indicated that trophic state in the ponds was cultural species specific. In fish ponds, soluble reactive phosphorus (SRP) was the major component of the total phosphorus (TP). Accumulation of P and imbalance of N and P ratio occurred due to exogenous inputs. Meanwhile, the concentrations of ammonium and nitrite were also the highest in the fish ponds, reaching 4.14 mg L-1 and 0.29 mg L-1 respectively. Enzymatic kinetic parameters were used to assess trophic state and regeneration rate of N and P in the ponds. The turnover time of organic P mediated by AP (T-AP) and its Michaelis constant (K-m) were found to be significantly positively correlated to SRP concentrations (P < 0.05) and negatively related to N and P ratios (P < 0.05). With increasing TP concentrations, the maximum velocity (V-max) of LAP increased linearly (P < 0.05), accelerating the efficiency of N regeneration. These results suggested excessive P limited the hydrolysis of organic P but promoted the production of harmful NH4+-N from organic N, implying that extracellular enzyme balanced regenerations of N and P in the ponds. Analysis of sediment suggested that there existed higher risks of benthic P release in fish ponds, while the equilibrium P concentrations (EPC0) were significantly positively related to proportion of Fe (OOH) similar to P, contents of TP and organic carbon (OC) (P < 0.05). Even so, the sediment tended to adsorb phosphate rather than release P in most farming months. Overall, harmful ammonium and nitrite accumulated massively in fish ponds due to high P loads, while massive P might derive from exogenous inputs or endogenous sources such as sediment. This study uncovered the coupling interactions between N and P cycles in traditional aquaculture ponds, implicating that a balanced N and P management measures were recommended in aquaculture ponds during farming period.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 水产品种创制与高效养殖全国重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
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  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅