论文
论文标题: Molecular cloning and functional analysis of polymeric immunoglobulin receptor, pIgR, gene in mandarin fish Siniperca chuatsi
作者: Ji, Jia Xiang; Zhang, Lin; Li, Li; Wang, Kai Lun; Hou, Jing; Liu, Lan Hao; Li, Bo; Zhang, Bai Dong; Li, Nan; Chen, Shan Nan; Nie, Pin
出版刊物: FISH & SHELLFISH IMMUNOLOGY
出版日期: JUN
出版年份: 2023
卷/期:
DOI: 10.1016/j.fsi.2023.108732
论文摘要: Polymeric immunoglobulin receptor (pIgR) can bind and transport immunoglobulins (Igs), thus playing a role in mucosal immunity. In this study, pIgR gene was cloned in mandarin fish, Siniperca chuatsi, with the open reading frame (ORF) of 1011 bp, encoding 336 amino acids. The pIgR protein consists of a signal peptide, an extracellular domain, a transmembrane domain and an intracellular region, with the presence of two Ig-like domains (ILDs) in the extracellular domain, as reported in other species of fish. The pIgR gene was expressed in all organs/tissues of healthy mandarin fish, with higher level observed in liver and spleen. Following the immersion infection of Flavobacterium columnare, pIgR transcripts were detected in immune related, especially mucosal tissues, with significantly increased transcription during the first two days of infection. Through transfection of plasmids expressing pIgR, IgT and IgM, pIgR was found to be interacted with IgT and IgM as revealed by coimmunoprecipitation and immunofluorescence.
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  • 水生生物博物馆
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