论文
论文标题: Functional characterization of IL-18 receptor subunits, IL-18R? and IL-18R?, and its natural inhibitor, IL-18 binding protein (IL-18BP) in rainbow trout Oncorhynchus mykiss
作者: Yang, Yue Chong; Chen, Shan Nan; Gan, Zhen; Huang, Lin; Li, Nan; Wang, Kai Lun; Nie, P.
出版刊物: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期: MAR
出版年份: 2023
卷/期:
DOI: 10.1016/j.dci.2022.104610
论文摘要: As an important proinflammation and immunomodulatory cytokine, IL-18 has been reported in several species of fish, but its receptor subunits, IL-18R alpha and IL-18R beta, and its decoy receptor, IL-18BP, have not been functionally characterized in fish. In the present study, IL-18R alpha, IL-18R beta and IL-18BP were cloned from rainbow trout Oncorhynchus mykiss, and they possess common conserved domains with their mammalian orthologues. In tested organs/tissues, IL-18R alpha and IL-18R beta exhibit basal expression levels, and IL-18BP has a pattern of constitutive expression. When transfected with different combinations of chimeric receptors in HEK293T cells, recombinant IL-18 (rIL-18) can induce the activation of NF-kappa B only when pcDNA3.1-IL-18R alpha/IL-1R1 and pcDNA3.1-IL-18R beta/ IL-1RAP were both expressed. On the other hand, recombinant receptors, including rIL-18BP, rIL-18R alpha-ECD-Fc and rIL-18R beta-ECD-Fc can down-regulate significantly the activity of NF-kappa B, suggesting the participation of IL18R alpha, IL-18R beta and IL-18BP in rainbow trout IL-18 signal transduction. Co-IP assays indicated that IL-18R beta may form a complex with MyD88, IRAK4, IRAK1, TRAF6 and TAB2 in HEK293T cells, indicating that IL-18R beta, in IL 18 signalling pathway, is associated with these signalling molecules. In conclusion, IL-18R alpha, IL-18R beta and IL-18BP in rainbow trout are conserved in function and signalling pathway with their mammalian orthologues.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 淡水生态与生物技术国家重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
  • 农业农村部
  • 科学技术部
  • 生态环境部
  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅