论文
论文标题: Characterization of CD166 from Nile tilapia (Oreochromis niloticus) displays a broad pathogen recognition spectrum and involved the immune response to microbial aggression
作者: Wang, Zhiwen; Xie, Caixia; Li, Yuan; Wang, Bei; Jian, Jichang; Lu, Yishan; Xia, Liqun; Chang, Mingxian
出版刊物: FISH & SHELLFISH IMMUNOLOGY
出版日期: MAR
出版年份: 2023
卷/期:
DOI: 10.1016/j.fsi.2023.108516
论文摘要: CD166 is a member of the immunoglobulin superfamily of cell adhesion molecules, and its mediated adhesion plays a crucial role in different physiological and pathological phenomena, especially related to leukocyte extravasation, immune synapse stability, T cell activation and proliferation. In this study, CD166 was identified from Nile tilapia (Oreochromis niloticus, OnCD166). OnCD166 contains an open reading frame of 1671 bp that encodes a peptide of 556 amino acids, and contains five consecutive extracellular immunoglobulin domains. It's tissue distribution and expression patterns after S. agalactiae challenge were also investigated. OnCD166 is widely distributed in various tissues of healthy tilapia. After Streptococcus agalactiae challenge, OnCD166 expressions were significantly up-regulated in all tested immune tissues. Meanwhile, the recombinant OnCD166 (rOnCD166E) protein showed strong agglutinating activities against both Gram-negative bacteria and Gram-positive bacteria. Moreover, rOnCD166E could promote phagocytosis of macrophages. Taken together, our re-sults illustrated that OnCD166 might as a receptor involved in the immune recognition and phagocytosis against invading pathogen, which play important roles in the immune responses of Nile tilapia against bacterial pathogens.
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  • 中国科学院藻类生物学重点实验室
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  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
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  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
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  • 水生生物博物馆
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