论文
论文标题: Dietary sanguinarine ameliorates growth impairment and intestinal dysfunction in Ctenopharyngodon idellus fed a high cottonseed meal diet
作者: Shi, Yong; Liu, Yuanxiang; Xie, Kai; Dai, Jihong; Wang, Ya; Xie, Shouqi; Hu, Yi; Zhong, Lei
出版刊物: AQUACULTURE
出版日期: NOV 15
出版年份: 2023
卷/期:
DOI: 10.1016/j.aquaculture.2023.739864
论文摘要: High cottonseed meal diets can reduce the growth and cause intestinal damage of aquatic animals. Sanguinarine is a plant alkaloid with demonstrated anti-inflammatory. Howerve, its role in the negative effects caused the high cottonseed meal diet is unclear. Herein, an eight-week feeding trial was conducted to evaluate the effects of sanguinarine supplementation in a high cottonseed meal diet on the growth and intestinal health (such as inflammation, physical barrier, oxidative stress, and microbiota of Ctenopharyngodon idellus, providing a theoretical basis for the application of sanguinarine in cottonseed meal diets. A basal diet of 0% cottonseed meal was designated as the control diet (CON). Diets (44% cottonseed meal) supplemented with four sanguinarine (0, 300, 600, and 1200 & mu;g/kg; designated as the CM, CMLS, CMMS, and CMHS, respectively) were prepared. There were 3 replicates per group and 50 fish per replicate. One-way ANOVA and Duncan's multiple comparisons of the means were used in this experiment. The results showed that feeding grass carp with CM obviously reduced the growth, impaired the intestinal physical and immune barrier function, and disrupted the microbiota balance of the intestine. Compared with the CM group, supplemented sanguinarine markedly improved the growth performance, serum AKP, C3, and IgM levels of grass carp, and enhanced intestinal antioxidant capacity (gpx1, cat, and gsto expression levels obviously up-regulated and ROS and MDA contents reduced). Sanguinarine observably downregulated the expression levels of il-1 & beta;, il-8, il-6, and tnf-& alpha; and up-regulated the expression levels of il-10, il-15, and tgf-& beta;1 by inhibiting NF-& kappa;B signaling molecules, thereby alleviating intestinal inflammation caused by a CM diet. Additionally, sanguinarine observably down-regulated claudin-12 and claudin-15 gene expression and upregulated zo-1 and occludin gene expression by inhibiting MLCK signaling molecules. Besides, compared with the CM group, the & alpha;-diversity indices and abundances of Proteobacteria, Actinobacteria, and Planctomycetes in the CMMS group were markedly reduced, while the abundance of Firmicutes was the opposite. In conclusion, the CM diet negatively impacted grass carp, and supplemented sanguinarine can improve growth, enhance intestinal antioxidant ability, alleviate intestinal barrier damage, and ameliorate intestinal microbiota homeostasis.
== 实验室与学会 ==
  • == 实验室与学会 ==
  • 淡水生态与生物技术国家重点实验室
  • 中国科学院藻类生物学重点实验室
  • 农业部淡水养殖病害防治重点实验室
  • 武汉白暨豚保护基金会
  • 湖北省海洋湖沼学会
  • 中国动物学会原生动物学分会
  • 中国动物学会斑马鱼分会
  • 湖北省暨武汉动物学会
  • 中国水产学会鱼病学专业委员会
  • 中国鱼类学会
== 平台建设 ==
  • == 平台建设 ==
  • “一带一路”海域赤潮数据库
  • 国家水生生物种质资源库
  • 国家斑马鱼资源中心
  • 中国科学院淡水藻种库
  • 中国科学院武汉生命科学大型仪器区域中心
  • 湿地生态系统观测研究野外站联盟
  • 中国科学院水生生物研究所分析测试中心
  • 中国科学院超级计算武汉分中心
  • 水生生物博物馆
== 相关网站推荐 ==
  • == 相关网站推荐 ==
  • 中国科学院
  • 农业农村部
  • 科学技术部
  • 生态环境部
  • 国家自然科学基金委员会
  • 中国科学院武汉分院
  • 湖北省科学技术厅
  • 湖北省生态环境厅
  • 湖北省农业农村厅