| 论文摘要： |
A Gram- stain- negative, red pigment- producing, aerobic, and rod- shaped bacterial strain (A2- 2T) was isolated from a bleached scleractinian coral (Porites lutea). Strain A2- 2T grew with 1.0-7.0 % (w/v) NaCl (optimum, 3.0 %), at pH 6.0-11.0 (optimum, pH 8.0), and at 18-41 degrees C (optimum, 35 degrees C). Results of phylogenetic analysis based on 16S rRNA gene sequences suggested that strain A2-2T fell within the genus Spartinivicinus and was closely related to Spartinivicinus ruber S2-4-1HT (98.1 % sequence similarity) and Spartinivicinus marinus SM1973T (98.0 % sequence similarity). The predominant cellular fatty acids of strain A2- 2T were C16: 0 (31.0 %), summed feature 3 (29.0 %), summed feature 8 (11.7 %), C12: 0 3- OH (6.4 %), and C10: 0 3- OH (5.5 %), while the major respiratory quinone was Q- 9. The polar lipids mainly comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unidentified phospholipid. The genome size of strain A2- 2T was 6.8 Mb, with a G+C content of 40.2 mol%. The DNA-DNA hybridization value was 24.2 % between A2- 2T and S. ruber S2-4-1HT and 36.9 % between A2- 2T and S. marinus SM1973T, while the average nucleotide identity values were 80.1 and 88.8 %, respectively. Based on these findings, strain A2- 2T could be recognized to represent a novel species of the genus Spartinivicinus, for which the name Spartinivicinus poritis sp. nov. is proposed. The type strain is A2- 2T (=MCCC 1K08228T=KCTC 8323T). |
