| 论文摘要： |
Sinipercachuatsi rhabdovirus (SCRV) has caused severe disease and mortality in commercial fish in China. To investigate the molecular relationships between pathogen and host, transcriptome analysis of SCRV-infected and non-infected E11 cells was obtained using the Illumina HiSeq X Ten sequencing system. A total of 29,894 unigenes were assembled and a total of 4137 differentially expressed genes (DEGs), consisting of 2189 up-regulated and 1948 down-regulated unigenes, were detected between SCRV-infected and non-infected E11 cells. To understand the immune response of the host against the virus, pathway function analysis indicates that SCRV infection affected 31 typical immune-related pathways, including MAPK signaling pathway, PI3K-Akt signaling pathway, endocytosis, and apoptosis, suggesting that E11 cells resist SCRV invasion through these innate immune pathways. Overall, qRT-PCR results showed similar expression to Illumina HiSeq, with reliable sequencing results. These results provided some vital clues which will be helpful for future study on SCRV-host interactions. |
